Xylitol, an anticaries agent, exhibits potent inhibition of inflammatory responses in human THP-1-derived macrophages infected with Porphyromonas gingivalis.
Xylitol, an anticaries agent, exhibits potent inhibition of inflammatory responses in human THP-1-derived macrophages infected with Porphyromonas gingivalis.
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DOI：
10.1902/jop.2014.130455
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发表时间：
2014-06
影响因子：
4.3
通讯作者：
Chung J
中科院分区：
文献类型：
--
作者：
Park E;Na HS;Kim SM;Wallet S;Cha S;Chung J
木糖醇是一种众所周知的防蛀剂，已被用于预防和治疗龋齿。在这项研究中，木糖醇的抗炎作用评估可能用于预防和治疗牙周感染。用活的牙龈卟啉单胞菌刺激THP-1（人单核细胞系）来源的巨噬细胞中细胞因子的表达，并使用酶联免疫吸附法和商用多重检测试剂盒检测木糖醇对牙龈卟啉单胞菌诱导的活细胞因子产生的影响。采用吞噬实验、活细胞计数和Griess试剂测定木糖醇对细胞吞噬和一氧化氮生成的影响。免疫染色法检测木糖醇对牙龈假单胞菌黏附的影响，流式细胞术检测共刺激分子的表达。活的牙龈假单胞菌感染增加了代表性的促炎细胞因子的产生，如肿瘤坏死因子-α和白细胞介素(IL)-1β，以多种感染和时间依赖的方式。活的牙龈假单胞菌也增加了细胞因子和趋化因子的释放，如IL-12 p40、eotaxin、干扰素γ诱导蛋白10、单核细胞趋化蛋白-1和巨噬细胞炎症蛋白-1。木糖醇预处理能显著抑制牙龈假单胞菌诱导的细胞因子和一氧化氮的产生。此外，木糖醇还能抑制活牙龈卟啉卟啉在thp -1来源的巨噬细胞上的附着。此外，木糖醇对大肠杆菌和牙龈卟啉单胞菌均具有抗吞噬活性。这些发现表明木糖醇在活牙龈卟啉卟啉感染的thp -1来源的巨噬细胞中起抗炎作用，这支持了木糖醇在牙周炎中的应用。
Xylitol is a well-known anticaries agent and has been used for the prevention and treatment of dental caries. In this study, the anti-inflammatory effects of xylitol are evaluated for possible use in the prevention and treatment of periodontal infections. Cytokine expression was stimulated in THP-1 (human monocyte cell line)-derived macrophages by live Porphyromonas gingivalis, and enzyme-linked immunosorbent assay and a commercial multiplex assay kit were used to determine the effects of xylitol on live P. gingivalis–induced production of cytokine. The effects of xylitol on phagocytosis and the production of nitric oxide were determined using phagocytosis assay, viable cell count, and Griess reagent. The effects of xylitol on P. gingivalis adhesion were determined by immunostaining, and costimulatory molecule expression was examined by flow cytometry. Live P. gingivalis infection increased the production of representative proinflammatory cytokines, such as tumor necrosis factor-α and interleukin (IL)-1β, in a multiplicity of infection– and time-dependent manner. Live P. gingivalis also enhanced the release of cytokines and chemokines, such as IL-12 p40, eotaxin, interferon γ–induced protein 10, monocyte chemotactic protein-1, and macrophage inflammatory protein-1. The pretreatment of xylitol significantly inhibited the P. gingivalis– induced cytokines production and nitric oxide production. In addition, xylitol inhibited the attachment of live P. gingivalis on THP-1-derived macrophages. Furthermore, xylitol exerted anti-phagocytic activity against both Escherichia coli and P. gingivalis. These findings suggest that xylitol acts as an antiinflammatory agent in THP-1-derived macrophages infected with live P. gingivalis, which supports its use in periodontitis.