TRANSFORMING CAPACITIES OF AVIAN ERYTHROBLASTOSIS VIRUS MUTANTS DELETED IN THE ERBA OR ERBB ONCOGENES
TRANSFORMING CAPACITIES OF AVIAN ERYTHROBLASTOSIS VIRUS MUTANTS DELETED IN THE ERBA OR ERBB ONCOGENES
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DOI：
10.1016/0092-8674(83)90513-5
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
VENNSTROM, B
中科院分区：
文献类型：
--
作者：
FRYKBERG, L;PALMIERI, S;VENNSTROM, B
禽成红细胞增多症病毒（AEV）的突变体是通过删除病毒癌基因v-erbA和v-erbB中的大核苷酸片段而构建的。erbA突变体（erbA-B+）保留了体外转化成纤维细胞的能力，这些细胞表现出大多数典型的野生型AEV转化成纤维细胞的转化特征。突变体在感染培养物中的骨髓细胞后诱导小的红系集落。接种erbA-B+病毒或erbA-B+转化细胞的鸡发生肉瘤或非典型红细胞白血病。由erbA-B+病毒在体内或体外转化的红系细胞在分化中似乎不像野生型转化细胞那样被紧密地阻断。感染erbA+B-突变体的成纤维细胞在所有测试的转化参数中类似于正常细胞，并且未观察到突变体的骨髓细胞转化。结果表明，AEV的主要转化特性由erbB编码，erbA增强了AEV的转化作用。
Mutants of avian erythroblastosis virus (AEV) were constructed by deleting large nucleotide segments in each of the viral oncogenes termed v-erbA and v-erbB. Mutants in erbA (erbA-B+) retained the ability to transform fibroblasts in vitro, and these cells exhibited most of the transformation characteristics that typify wild-type AEV-transformed fibroblasts. The mutants induced small erythroid colonies upon infection of bone marrow cells in culture. Chickens inoculated with erbA-B+ virus or with erbA-B+-transformed cells developed sarcomas or atypical erythroid leukemias. The erythroid cells transformed in vivo or in vitro by the erbA-B+ viruses appeared not to be as tightly blocked in differentiation as wild-type transformed cells. Fibroblasts infected with the erbA+B- mutant resembled normal cells in all transformation parameters tested, and no bone marrow cell transformation was observed with the mutant. The results indicate that the main transforming properties of AEV are encoded in erbB and that its effects are enhanced by erbA.