Ex vivo enrichment of malignant carcinoma cells in primary culture
Ex vivo enrichment of malignant carcinoma cells in primary culture
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DOI：
10.1080/00313020701569949
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发表时间：
2007-01-01
期刊：
影响因子：
4.5
通讯作者：
Silverman, Jan F.
中科院分区：
文献类型：
--
作者：
Heinzman, Jamie M.;Brower, Stacey L.;Silverman, Jan F.
目的：在原代培养中建立和维持人类肿瘤可能是具有挑战性的。在这种应用中，需要一个短期的原代培养过程，以确保在培养中保持的细胞代表体内肿瘤，以进行化学反应测试。为了确保培养出合适的细胞，必须对培养物进行恶性程度评估。临床判断恶性肿瘤的金标准是由细胞病理学专家进行细胞学评价。方法：建立50例人肿瘤标本(乳腺、结肠、肺、卵巢)，并进行原代培养。细胞旋体在培养开始时制备，并在培养过程完成时再次制备。结果：86%(43/50)的培养物恶性细胞百分率增加，8%(4/50)的培养物在整个培养过程中保持不变的恶性细胞百分率，6%(3/50)的培养物恶性细胞百分率下降。恶性细胞百分率平均增加37%，且与培养时间(5-28天)无关。结论：所描述的原代培养过程对恶性细胞具有丰富的作用，可用于进一步的评价，如化学反应试验。
Aims: Establishing and maintaining human tumours in primary culture can be challenging. In this application, a short-term primary culture process is desired to ensure cells maintained in culture are representative of the in vivo tumour for the purpose of chemoresponse testing. To ensure the appropriate cells are being grown, the cultures must be evaluated for malignancy. The clinical gold standard determination of malignancy is cytological evaluation by a cytopathologist.Methods: Fifty human tumour specimens (breast, colon, lung, ovary) were established and maintained in primary culture. Cytospins were prepared upon initiation of culture and again at completion of the culture process. Cytospins were stained (Diff- Quik, Papanicolaou) and evaluated by a cytopathologist for the percentage of malignant cells at both times.Results: An increase in the percentage of malignant cells was noted in 86% (43/50) of the cultures evaluated; 8% (4/ 50) of the cultures maintained the same percentage of malignant cells throughout the culture period, and 6% (3/50) displayed a decrease in malignant cells. On average, the percentage of malignant cells increased by 37% and was not associated with the length of culture (range 5-28 days).Conclusions: The described primary culture process enriches for malignant cells, which is desirable for further evaluation such as chemoresponse testing.