RELATIONSHIP BETWEEN SPERM TRANSPORT AND PENETRATION OF EGGS IN RABBIT OVIDUCT
RELATIONSHIP BETWEEN SPERM TRANSPORT AND PENETRATION OF EGGS IN RABBIT OVIDUCT
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DOI：
10.1093/biolreprod/8.4.441
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发表时间：
1973-01-01
影响因子：
3.6
通讯作者：
HARPER, MJK
中科院分区：
文献类型：
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作者：
HARPER, MJK
从注射HCG诱导排卵后不久的供兔身上回收的未受精卵，在已知时间将其转移到已交配受者的输卵管中或用2亿个精子受精并注射HCG的受者的输卵管中。研究发现，在交配后9-9.75 h将卵转移到交配受体后的1.5 h内，只有40%的卵被穿透，而在交配后13.75-14.25 h和19.5-20.0 h将卵转移到交配受体后的1.5 h内，分别有58%和66%的卵被穿透。在交配后24小时，卵子穿透率再次下降到43%。在子宫输卵管连接处结扎输卵管表明，在人工授精后9.25-9.75小时，输卵管中有足够的精子，以确保在人工授精后14或17小时（而不是20小时）转移后的这种增强受精。这些结果表明，尽管精子继续从子宫迁移到输卵管，但输卵管中更多的精子在后来的时间里并不是造成这种现象的原因。在壶腹-峡交界处或峡中部进行结扎的进一步实验表明，精子在子宫输卵管交界处上方的峡近端保留了几个小时。固定整条输卵管，连续切片，测定精子数量和分布，发现在授精后9 h，受精部位精子较少。在受精后14或20小时杀死的动物的输卵管中，输卵管中的精子（不管子宫输卵管连接处是否结扎）已经重新分布，在受精部位发现了更多的精子。由此得出结论，由于卵子和精子有可能立即相遇，因此在较晚移植后的短时间内（1.5 h）有更多的卵子穿透。
Unfertilized eggs recovered from donor rabbits shortly after ovulation induced by injection of HCG were transferred to the oviducts of mated recipients or recipients inseminated with 200 million spermatozoa and injected with HCG at known times. It was found that although only 40% of such eggs were penetrated in 1.5 h following transfer to mated recipients at 9–9.75 h after mating, 58 and 66% were penetrated during the same period if transfers were delayed until 13.75–14.25 and 19.5–20.0 h after mating, respectively. By 24 h after mating the percentage of eggs penetrated had fallen again to 43%. It was shown by ligation of the oviduct at the uterotubal junction that adequate sperm were in the oviduct by 9.25–9.75 h after insemination to ensure this enhanced fertilization following transfer at 14 or 17 h but not 20 h after insemination. These results implied that although sperm continued to migrate from the uterus into the oviduct, more sperm in the oviduct at later times was not responsible for this phenomenon. Further experiments in which ligation was done at the ampullar-isthmic junction or the middle of the isthmus showed that sperm were retained in the proximal isthmus just above the uterotubal junction for several hours. When whole oviducts were fixed and serially sectioned and the number and distribution of sperm determined, it was found that at 9 h after insemination few sperm were at the site of fertilization. In the oviducts of animals killed at 14 or 20 h after insemination, the sperm in the oviduct (irrespective of whether the uterotubal junction had been ligated or not) had become redistributed with many more found at the site of fertilization. It is concluded that more eggs are penetrated in a short period of time (1.5 h) followed late transfers because there is the immediate possibility of egg and sperm meeting.