Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.
Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.
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DOI:
10.1002/chem.201505143
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发表时间:
2016-03-24
期刊:
影响因子:
--
通讯作者:
Bode BE
中科院分区:
文献类型:
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作者:
Valera S;Ackermann K;Pliotas C;Huang H;Naismith JH;Bode BE
Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron–electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane‐embedded ion channels where data analysis is commonly hindered by multi‐spin effects.