Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.

Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.
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DOI:
10.1002/chem.201505143
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发表时间:
2016-03-24
期刊:
Chemistry (Weinheim an der Bergstrasse, Germany)
影响因子:
--
通讯作者:
Bode BE
Bode BE
中科院分区:
其他
文献类型:
--
作者:
Valera S;Ackermann K;Pliotas C;Huang H;Naismith JH;Bode BE

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脉冲电子顺磁共振(EPR)在结构生物学中的地位日益重要。脉冲电子-电子双共振(PELDOR)方法可以在纳米尺度上提取距离信息。在这里,我们展示了从多聚体系统中有效地提取距离,例如膜嵌入的离子通道,在这些系统中,数据分析通常受到多自旋效应的阻碍。
Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron–electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane‐embedded ion channels where data analysis is commonly hindered by multi‐spin effects.