Tmem16b is Specifically Expressed in the Cilia of Olfactory Sensory Neurons
Tmem16b is Specifically Expressed in the Cilia of Olfactory Sensory Neurons
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DOI：
10.1093/chemse/bjq007
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发表时间：
2010-03-01
期刊：
影响因子：
3.5
通讯作者：
Neuhaus, Eva M.
中科院分区：
文献类型：
--
作者：
Rasche, Sebastian;Toetter, Bastian;Neuhaus, Eva M.
钙激活氯离子通道(CaCCs)参与包括感觉信号转导在内的多种生理过程，但目前对其结构和功能知之甚少。我们对嗅觉上皮(OE)膜蛋白质组进行了蛋白质组学分析，并确定到目前为止尚未鉴定的膜蛋白是候选通道。嗅觉神经元(OSNs)中含量最丰富的膜蛋白之一是Tem16b，它是最近发现的CaCCs家族的成员。除了以前对Tem16b的研究外，我们在这里还表明Tem16b的表达对OE具有高度的特异性，而与之密切相关的TMEM16A在分泌上皮细胞中显示了广泛的表达模式。Native Tem16b定位于OSNs纤毛内，这与以往的电生理记录一致。
Calcium-activated chloride channels (CaCCs) are involved in many physiological processes, including sensory signal transduction, but only little is known to date about their structure and function. We performed a proteome analysis of the olfactory epithelium (OE) membrane proteome and identified so far uncharacterized membrane proteins as candidate channels. One of the most abundant membrane proteins in olfactory sensory neurons (OSNs) was Tmem16b, a member of a recently identified family of CaCCs. In addition to former studies performed on Tmem16b, we show here that Tmem16b expression is highly specific for the OE, in contrast to the closely related Tmem16a, which shows a broad expression pattern in secretory epithelial cells. Native Tmem16b is localized in the cilia of the OSNs, which is in agreement with previous electrophysiological recordings.