Spontaneous skin ulceration and defective T cell function in CD18 null mice.
Spontaneous skin ulceration and defective T cell function in CD18 null mice.
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CD18无效小鼠的自发皮肤溃疡和T细胞功能缺陷。
DOI：
10.1084/jem.188.1.119
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发表时间：
1998-07-06
期刊：
影响因子：
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通讯作者：
Beaudet AL
中科院分区：
文献类型：
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作者：
Scharffetter-Kochanek K;Lu H;Norman K;van Nood N;Munoz F;Grabbe S;McArthur M;Lorenzo I;Kaplan S;Ley K;Smith CW;Montgomery CA;Rich S;Beaudet AL
在小鼠中制备了白细胞整合素的β 2亚基CD 18的无效突变。纯合子CD18缺失小鼠发生慢性皮炎，伴有广泛的面部和下颌下糜烂。表型包括中性粒细胞计数升高、免疫球蛋白水平升高、淋巴结病、脾肿大和皮肤、淋巴结、肠道和肾脏中大量浆细胞。在自发发生的皮肤病变或诱发的毒性皮炎中发现极少数中性粒细胞。在CD18无效小鼠的活体显微镜检查显示，缺乏坚定的中性粒细胞附着到小静脉在提睾肌响应N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸。在T细胞增殖中发现一个严重的缺陷，在CD18空小鼠T细胞受体刺激时，无论是由葡萄球菌肠毒素A或主要组织相容性复合物同种异体抗原证明更大的作用，CD11/CD18整合素在T细胞反应比以前记录。裸小鼠可用于描述CD 18在体内的功能。
A null mutation was prepared in the mouse for CD18, the β2 subunit of leukocyte integrins. Homozygous CD18 null mice develop chronic dermatitis with extensive facial and submandibular erosions. The phenotype includes elevated neutrophil counts, increased immunoglobulin levels, lymphadenopathy, splenomegaly, and abundant plasma cells in skin, lymph nodes, gut, and kidney. Very few neutrophils were found in spontaneously occurring skin lesions or with an induced toxic dermatitis. Intravital microscopy in CD18 null mice revealed a lack of firm neutrophil attachment to venules in the cremaster muscle in response to N-formyl- methionyl-leucyl-phenylalanine. A severe defect in T cell proliferation was found in the CD18 null mice when T cell receptors were stimulated either by staphylococcal enterotoxin A or by major histocompatibility complex alloantigens demonstrating a greater role of CD11/CD18 integrins in T cell responses than previously documented. The null mice are useful for delineating the functions of CD18 in vivo.