The effect of irreversible electroporation on blood vessels
The effect of irreversible electroporation on blood vessels
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DOI：
10.1177/153303460700600407
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发表时间：
2007-08-01
影响因子：
2.8
通讯作者：
Rubinsky, Boris
中科院分区：
文献类型：
--
作者：
Maor, Elad;Ivorra, Antoni;Rubinsky, Boris
我们提出了一项关于不可逆电穿孔(IRE)对大血管的长期影响的初步研究。这项研究的动机是预期使用IRE治疗毗邻大血管的癌症肿瘤。将10个3800V/cm、100mU S的直流电IRE脉冲序列以每秒10个脉冲的频率直接作用于6只大鼠的颈动脉。在手术过程中测量组织电导率，正如预测的那样，在施加脉冲期间电导率增加，这表明这种测量可以用于控制IRE的应用。所有的动物都在手术中存活下来，并且没有表现出副作用。术后28d行组织学检查，血管结缔组织保持完整，动脉壁血管平滑肌细胞(VSMC)数量减少，未见动脉瘤、血栓形成或坏死。IRE消融后血管平滑肌细胞平均密度显著低于对照组(24±11比139±14，P<；0.001)，对细胞外基质成分和结构无明显损伤。除了这项研究与大血管附近癌症的治疗相关外，这些发现还初步表明，IRE可能用于治疗病理过程，其中希望减少VSMC群体的增殖，如再狭窄，并用于减轻临床重要部位的动脉粥样硬化过程，如冠状动脉、颈动脉和肾动脉。
We present a pilot study on the long term effects of irreversible electroporation (IRE) on a large blood vessel. The study was motivated by the anticipated use of IRE for treatment of cancer tumors abutting large blood vessels. A sequence of 10 direct current IRE pulses of 3800 V/cm, 100 mu s each, at a frequency of 10 pulses per second, were applied directly to the carotid artery in six rats. Measuring tissue conductivity during the procedure showed, as predicted, an increase in conductivity during the application of the pulse, which suggests that this measurement can be used to control the application of IRE. All the animals survived the procedure and showed no side effects. Histology performed 28 days after the procedure showed that the connective matrix of the blood vessels remained intact and the number of vascular smooth muscle cells (VSMC) in the arterial wall decreased with no evidence of aneurysm, thrombus formation or necrosis. Average VSMC density was significantly lower following IRE ablation compared with control (24 +/- 11 vs. 139 +/- 14, P < 0.001), with no apparent damage to extra cellular matrix components and structure. In addition to the relevance of this study to treatment of cancer near large blood vessels these findings tentatively suggest that IRE has possible applications to treatment of pathological processes in which it is desired to reduce the proliferation of VSMC population, such as restenosis and for attenuating atherosclerotic processes in clinical important locations such as coronary, carotid and renal arteries.