Antimicrobial activity of kaempferol and resveratrol in binary combinations with parabens or propyl gallate against Enterococcus faecalis
Antimicrobial activity of kaempferol and resveratrol in binary combinations with parabens or propyl gallate against Enterococcus faecalis
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DOI：
10.1016/j.foodcont.2015.10.001
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发表时间：
2016-03-01
期刊：
影响因子：
6
通讯作者：
Rua, Javier
中科院分区：
文献类型：
--
作者：
del Valle, Pilar;Rosario Garcia-Armesto, Maria;Rua, Javier
采用棋盘法和时间杀灭法研究了2种天然酚类化合物（白藜芦醇和山萘酚）和3种人工合成酚类化合物（没食子酸丙酯、尼泊金丙酯和尼泊金庚酯）对2株粪肠球菌的抗菌活性。羟苯甲酸庚酯是最有效的，其次是白藜芦醇，山奈酚，没食子酸丙酯和羟苯甲酸丙酯，根据其最小抑制浓度（MIC）范围为13.4 μ g/mL（-1）（对羟基苯甲酸庚酯）至881.82 μ g mL（-1）（对羟基苯甲酸丙酯）：山奈酚加白藜芦醇和二元组合的每一个与丙基没食子酸酯或对羟基苯甲酸酯显示没有交互作用的棋盘法。山奈酚和白藜芦醇组合在阳离子调节的Mueller-Hinton肉汤和脱脂乳中产生生长抑制，其特征在于滞后期增加和最大比生长速率降低。尼泊金庚酯单独或与山奈酚组合显示抑菌作用。山奈酚与白藜芦醇、没食子酸丙酯、对羟基苯甲酸丙酯或庚酯组合产生抗氧化活性的拮抗作用，使用两种对羟基苯甲酸酯获得最高效果（约减少25%）。再生机制分析的拮抗作用进行了讨论的二元组合测试对两株大肠杆菌的抗菌活性的增加。粪便(C)2015爱思唯尔有限公司版权所有。
Antimicrobial activity of two natural phenolic compounds (resveratrol and kaempferol) and three synthetic (propyl gallate, propyl- and heptyl paraben) against two strains of Enterococcus faecalis were analyzed by checkerboard and time-kill methods. Heptyl paraben was the most effective followed by resveratrol, kaempferol, propyl gallate and propyl paraben, according to their minimal inhibitory concentrations (MICs) ranging from 13.4 mu g mL(-1) (heptyl paraben) to 881.82 mu g mL(-1) (propyl paraben): Kaempferol plus resveratrol and binary combinations of each one with propyl gallate or parabens showed no interaction effects by the checkerboard method. Kaempferol and resveratrol in combination produce a growth inhibition in cation adjusted Mueller-Hinton broth and in skimmed milk, characterized by an increase in the lag phase and a decrease in the maximum specific growth rate. Heptyl paraben alone or in combination with kaempferol showed bacteriostatic effects. Kaempferol combined with resveratrol, propyl gallate, propyl- or heptyl paraben produces antagonistic effect in antioxidant activity, obtaining the highest effect with the two parabens (approximately a 25% reduction). The antagonistic effects analyzed by regeneration mechanisms are discussed in relation to the increase of the antimicrobial activity shown by the binary combinations tested against the two strains of E. faecalis. (C) 2015 Elsevier Ltd. All rights reserved.