Comparison of miRNA Expression Profiles between HIV-1 and HIV-2 Infected Monocyte-Derived Macrophages (MDMs) and Peripheral Blood Mononuclear Cells (PBMCs).
Comparison of miRNA Expression Profiles between HIV-1 and HIV-2 Infected Monocyte-Derived Macrophages (MDMs) and Peripheral Blood Mononuclear Cells (PBMCs).
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DOI：
10.3390/ijms21186970
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发表时间：
2020-09-22
影响因子：
5.6
通讯作者：
Devadas K
中科院分区：
文献类型：
--
作者：
Biswas S;Chen E;Haleyurgirisetty M;Lee S;Hewlett I;Devadas K
在HIV-1感染的进展过程中，使用CCR 5共受体的嗜巨噬细胞HIV-1经历了对主要是嗜T细胞的CXCR 4的共受体使用的变化。这种共受体偏好的变化使病毒能够感染T细胞。已知HIV-2感染MDM和T细胞，并且是双嗜性的。本研究的目的是阐明宿主miRNAs的差异表达谱及其在HIV-1/HIV-2感染细胞中的作用。为了实现这一目标，在感染HIV-1/HIV-2的人PBMC和MDM中确定了比较性的总体miRNA表达谱。使用下一代测序（NGS）技术在HIV-1/HIV-2感染的PBMC和MDM中鉴定差异表达的miRNA。在感染HIV-1和HIV-2的MDM和PBMC中的比较性总体miRNA表达谱鉴定了几种宿主miRNA的差异表达。这些差异表达的miRNAs可能参与多种信号通路，如p53信号通路、PI 3 K-Akt信号通路、MAPK信号通路、FoxO信号通路和病毒致癌作用。因此，比较研究HIV-1和HIV-2感染后MDM细胞和T细胞中宿主miRNA的差异表达将有助于我们识别能够区分HIV-1和HIV-2感染的独特生物标志物。
During the progression of HIV-1 infection, macrophage tropic HIV-1 that use the CCR5 co-receptor undergoes a change in co-receptor use to CXCR4 that is predominately T cell tropic. This change in co-receptor preference makes the virus able to infect T cells. HIV-2 is known to infect MDMs and T cells and is dual tropic. The aim of this study was to elucidate the differential expression profiles of host miRNAs and their role in cells infected with HIV-1/HIV-2. To achieve this goal, a comparative global miRNA expression profile was determined in human PBMCs and MDMs infected with HIV-1/HIV-2. Differentially expressed miRNAs were identified in HIV-1/HIV-2 infected PBMCs and MDMs using the next-generation sequencing (NGS) technique. A comparative global miRNA expression profile in infected MDMs and PBMCs with HIV-1 and HIV-2 identified differential expression of several host miRNAs. These differentially expressed miRNAs are likely to be involved in many signaling pathways, like the p53 signaling pathway, PI3K-Akt signaling pathways, MAPK signaling pathways, FoxO signaling pathway, and viral carcinogenesis. Thus, a comparative study of the differential expression of host miRNAs in MDMs and T cell in response to HIV-1 and HIV-2 infection will help us to identify unique biomarkers that can differentiate HIV-1 and HIV-2 infection.
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影响因子：
64.5
作者：
Brunet, A;Bonni, A;Greenberg, ME
通讯作者：
Greenberg, ME
影响因子：
4.5
作者：
Di Leva G;Piovan C;Gasparini P;Ngankeu A;Taccioli C;Briskin D;Cheung DG;Bolon B;Anderlucci L;Alder H;Nuovo G;Li M;Iorio MV;Galasso M;Santhanam R;Marcucci G;Perrotti D;Powell KA;Bratasz A;Garofalo M;Nephew KP;Croce CM
通讯作者：
Croce CM
影响因子：
20.3
作者：
Fujiwara, Mamoru;Takiguchi, Masafumi
通讯作者：
Takiguchi, Masafumi
影响因子：
64.8
作者：
EMBRETSON, J;ZUPANCIC, M;HAASE, AT
通讯作者：
HAASE, AT
影响因子：
3.3
作者：
Ahluwalia JK;Khan SZ;Soni K;Rawat P;Gupta A;Hariharan M;Scaria V;Lalwani M;Pillai B;Mitra D;Brahmachari SK
通讯作者：
Brahmachari SK