Flowery odor formation revealed by differential expression of monoterpene biosynthetic genes and monoterpene accumulation in rose (Rosa rugosa Thunb.)
Flowery odor formation revealed by differential expression of monoterpene biosynthetic genes and monoterpene accumulation in rose (Rosa rugosa Thunb.)
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玫瑰（Rosa rugosa Thunb.）中单萜生物合成基因的差异表达和单萜积累揭示了花香的形成
DOI：
10.1016/j.plaphy.2013.12.006
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发表时间：
2014-02-01
影响因子：
6.5
通讯作者：
Sheng, Lixia
中科院分区：
文献类型：
--
作者：
Feng, Liguo;Chen, Chen;Sheng, Lixia
玫瑰是一种重要的观赏和经济植物。本论文从红毛菊中分离了4个编码单萜生物合成途径中的1-脱氧-N-木酮糖-5-磷酸合成酶（DXS）、1-脱氧-D-木酮糖-5-磷酸还原异构酶（DXR）、醇酰基转移酶（AAT）和芳樟醇合成酶（LIS）的基因。用实时荧光定量PCR技术分析了这些基因在不同花发育时期和花器官不同部位的表达情况。此外，还全面分析了四个单萜合成基因的表达与主要挥发性单萜及其乙酸酯衍生物积累的关系。结果表明，RrDXS、RrDXR和RrLIS基因在红球藻发育过程中表达一致。从蕾期到枯萎期，RrDXS和RrLIS基因的总体表达量明显低于RrDXR和RrAAT基因。虽然RrDXS、RrDXR、RrAAT和RrLIS基因在R. rugosa花器官中，表达量差异显著。香茅醇、香叶醇、橙花醇、芳樟醇、乙酸香茅酯、乙酸香叶酯和乙酸橙花酯等挥发性单萜的组成和含量在花器官不同发育时期和部位的变化差异显著。在此基础上，我们认为RrDXR和RrAAT基因可能在红毛菊挥发性单萜的生物合成中起关键作用。rugosa花中，这两个基因是调控玫瑰芳香成分次生代谢的重要候选基因。(C)2013年Elsevier Masson SAS。All rights reserved.
Rosa rugosa is an important ornamental and economical plant. In this paper, four genes encoding 1-deoxy-n-xylulose-5-phosphate synthase (DXS), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), alcohol acyltransferase (AAT) and linalool synthase (LIS) involved in the monoterpene biosynthesis pathways were isolated from R. rugosa 'Tangzi', and the expression patterns of these genes in different flower development stages and different parts of floral organs were determined by real-time quantitative fluorescence PCR. Furthermore, a comprehensive analysis was carried out into the relationship between expression of four monoterpene synthesis genes and accumulation of main volatile monoterpenes and their acetic acid ester derivatives. The results showed that the genes RrDXS, RrDXR and RrLIS showed consistent expressions during the development process for R. rugosa flower from budding to withering stage, the overall expression levels of gene RrDXS and RrLIS were obviously lower as compared with those of gene RrDXR and RrAAT. Although the gene RrDXS, RrDXR, RrAAT and RrLIS were expressed in all parts of R. rugosa floral organs, the expression levels varied significantly. The variations in the constituent and content of volatile monoterpenes including citronellol, geraniol, nerol, linalool, citronelly1 acetate, geranyl acetate and neryl acetate at different development stages and parts of floral organs were significantly different. On this basis, we concluded that the gene RrDXR and RrAAT might play a key role in the biosynthesis of volatile monoterpenes in R. rugosa flowers, and the two genes are important candidate genes for the regulation of secondary metabolism for rose aromatic components. (C) 2013 Elsevier Masson SAS. All rights reserved.