Identification and functional characterization of the BBX24 promoter and gene from chrysanthemum in Arabidopsis
Identification and functional characterization of the BBX24 promoter and gene from chrysanthemum in Arabidopsis
复制标题
拟南芥菊花BBX24启动子及基因的鉴定及功能表征
DOI：
10.1007/s11103-015-0347-5
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发表时间：
2015-09-01
影响因子：
5.1
通讯作者：
Hong, Bo
中科院分区：
文献类型：
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作者：
Imtiaz, Muhammad;Yang, Yingjie;Hong, Bo
B-box（BBX）家族是拟南芥中锌指转录因子的一个亚组，其调节开花时间、光调节形态建成和非生物胁迫。在菊花中过量表达锌指转录因子CmBBX 24基因，可以提高菊花对非生物胁迫的耐受性。我们对CmBBX 24的启动子进行了研究和鉴定，分离了一个2.7-kb的CmBBX 24启动子序列，并注释了一些与非生物胁迫相关的顺式调控元件，如DRE，MYB，MYC，以及响应植物激素的顺式调控元件，如GARE，ABRE和CARE。我们还观察到一些与光相关的顺式元件，如TBOX和GBOX，以及一些组织特异性顺式元件，如保卫细胞（TAAAG）。CmBBX 24启动子的表达产生了明确的反应，在叶和根的反应较低，基于β-葡萄糖醛酸酶组织化学染色和荧光分析。通过非生物胁迫（甘露醇、低温）、激素（赤霉酸、脱落酸）和不同光处理（白色、蓝色、红色）诱导CmBBX 24启动子;通过荧光分析测量叶和根中的活化。从启动子的5 '端缺失片段导致在各种胁迫条件下的不同响应。一些CmBBX 24启动子片段被认为是更重要的比其他的调节所有的压力，而其他片段是相对更具体的压力类型。D 0-、D1-、D2-、D3-和D4-proCmBBX 24：：CmBBX 24转基因拟南芥品系被发现比对照更耐受低温和干旱胁迫。因此，我们推测，CmBBX 24是在拟南芥的非生物胁迫的调节和CmBBX 24启动子在非生物胁迫条件下是诱导的首要重要性。因此，我们认为CmBBX 24是一个潜在的候选人，用于重要的观赏植物育种计划。
The B-box (BBX) family is a subgroup of zinc finger transcription factors that regulate flowering time, light-regulated morphogenesis, and abiotic stress in Arabidopsis. Overexpression of CmBBX24, a zinc finger transcription factor gene in chrysanthemum, results in abiotic stress tolerance. We have investigated and characterized the promoter of CmBBX24, isolating a 2.7-kb CmBBX24 promoter sequence and annotating a number of abiotic stress-related cis-regulatory elements, such as DRE, MYB, MYC, as well as cis-elements which respond to plant hormones, such as GARE, ABRE, and CARE. We also observed a number of cis-elements related to light, such as TBOX and GBOX, and some tissue-specific cis-elements, such as those for guard cells (TAAAG). Expression of the CmBBX24 promoter produced a clear response in leaves and a lower response in roots, based on beta-glucuronidase histochemical staining and fluorometric analysis. The CmBBX24 promoter was induced by abiotic stresses (mannitol, cold temperature), hormones (gibberellic acid, abscisic acid), and different light treatments (white, blue, red); activation was measured by fluorometric analysis in the leaves and roots. The deletion of fragments from the 5'-end of the promoter led to different responses under various stress conditions. Some CmBBX24 promoter segments were found to be more important than others for regulating all stresses, while other segments were relatively more specific to stress type. D0-, D1-, D2-, D3-, and D4-proCmBBX24::CmBBX24 transgenic Arabidopsis lines developed for further study were found to be more tolerant to the low temperature and drought stresses than the controls. We therefore speculate that CmBBX24 is of prime importance in the regulation of abiotic stress in Arabidopsis and that the CmBBX24 promoter is inductive in abiotic stress conditions. Consequently, we suggest that CmBBX24 is a potential candidate for the use in breeding programs of important ornamental plants.