Membrane glycoprotein p150,95 of human cytotoxic T cell clone is involved in conjugate formation with target cells.
Membrane glycoprotein p150,95 of human cytotoxic T cell clone is involved in conjugate formation with target cells.
复制标题
人细胞毒性 T 细胞克隆的膜糖蛋白 p150,95 参与与靶细胞的缀合物形成。
作者：
G. Keizer;J. Borst;W. Visser;R. Schwarting;J. D. Vries;C. Figdor
p150,95异二聚体是白细胞功能相关抗原（LFA）家族的三种成员之一，在单核细胞、粒细胞、NK细胞和一小部分淋巴细胞中表达。我们现在报道p150,95糖蛋白在一些细胞毒性T细胞克隆中表达，并参与这些克隆介导的细胞溶解。两个CTL克隆，克隆JS-93 （CD3+ CD4+ CD8-）和克隆JS-102 （CD3+ CD4- CD8+）表达高水平的p150和p95，并被证明分别特异性靶向HLA-DR和HLA-A2。免疫沉淀后的二维凝胶电泳显示，从两个克隆中分离的p150,95分子没有异质性。此外，我们证实了针对p150,95的单克隆抗体（moab）可以抑制克隆JS-93和克隆JS-102的细胞毒活性（分别为50%和47%）。单细胞试验显示，抑制发生在偶联物形成的水平，而不是在致命打击的水平。在针对LFA-1的moab中获得了类似的结果（p170,95）。针对LFA-1和p150,95的moab抑制CTL活性和缀合物形成的能力是加性的，导致与针对这些分子的共同β链的moab相似的抑制百分比。结果表明，至少部分CTL克隆在细胞表面表达p150,95抗原，该分子与LFA-1一样，在效应细胞和靶细胞之间偶联形成。
The p150,95 heterodimer, one of three members of the leukocyte function associated antigen (LFA) family, is expressed by monocytes, granulocytes, NK cells, and a small percentage of lymphocytes. We now report that the p150,95 glycoprotein is expressed by some cytotoxic T cell clones and that it is involved in cell-mediated cytolysis by these clones. Two CTL clones, clone JS-93 (CD3+ CD4+ CD8-) and clone JS-102 (CD3+ CD4- CD8+) expressed high levels of p150,95 and were shown to be specifically directed against HLA-DR and HLA-A2, respectively. Immunoprecipitations followed by two-dimensional gel electrophoresis demonstrated no heterogeneity in the p150,95 molecule isolated from both clones. Furthermore, we demonstrated that monoclonal antibodies (moab) directed against p150,95 could inhibit the cytotoxic activity of both clone JS-93 and clone JS-102 (50% and 47%, respectively). Single cell assays revealed the inhibition to occur at the level of conjugate formation rather than at the level of the lethal hit. Similar results were obtained with moab directed against LFA-1 (p170,95). The capacity of the moab directed against LFA-1 and p150,95 to inhibit CTL activity and conjugate formation were additive, resulting in a similar percentage of inhibition as found with moab directed against the common beta-chain of these molecules. It is concluded that at least some CTL clones express the p150,95 antigen at their cell surface, and that this molecule, like LFA-1, acts at the level of conjugate formation between effector and target cells.