Conjugation of Hyaluronic Acid onto Surfaces via the Interfacial Polymerization of Dopamine to Prevent Protein Adsorption
Conjugation of Hyaluronic Acid onto Surfaces via the Interfacial Polymerization of Dopamine to Prevent Protein Adsorption
复制标题
通过多巴胺的界面聚合将透明质酸缀合到表面以防止蛋白质吸附
DOI：
10.1021/acs.langmuir.5b02320
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发表时间：
2015-11-10
期刊：
影响因子：
3.9
通讯作者：
He, Zhimin
中科院分区：
文献类型：
--
作者：
Huang, Renliang;Liu, Xia;He, Zhimin
在许多应用中，非常需要一种多功能、方便且经济高效的方法，可用于将防污材料接枝到不同的表面上。在这里，我们报告了通过多巴胺聚合和阴离子透明质酸（HA）在金基底上同时沉积的防污表面的一步制造。在附着高度均匀的聚多巴胺（PDA）/HA混合膜后，Au表面的水接触角从84.9度降低到24.8度。表面等离子体共振分析结果表明，Au-PDA/HA表面以超低或低量（4.8-31.7 ng/cm(2)）吸附牛血清白蛋白、溶菌酶、β-乳球蛋白、纤维蛋白原和豆浆溶液中的蛋白质。 PDA/HA 表面的亲水性和良好的防污性能归因于 HA 链可能通过 PDA 和 HA 之间的氢键连接到其上表面。同时，PDA和HA之间的静电斥力可能阻止了PDA的聚集，从而形成高度均匀的PDA/HA杂化膜，其上表面具有HA链（具有拉伸结构）。我们还开发了一种简单的方法，使用 NaOH 水溶液作为水解剂去除 PDA/HA 薄膜并回收 Au 基板。 Au表面未受损，并且PDA/HA薄膜可以在表面重新沉积，即使在10个这样的循环后，表面仍表现出良好的防污性能。最后，我们发现这种接枝方法适用于其他基材，包括环氧树脂、聚苯乙烯、玻璃和钢，因为PDA与这些基材具有很强的附着力。
A versatile, convenient, and cost-effective method that can be used for grafting antifouling materials onto different surfaces is highly desirable in many applications. Here, we report the one-step fabrication of antifouling surfaces via the polymerization of dopamine and the simultaneous deposition of anionic hyaluronic acid (HA) on Au substrates. The water contact angle of the Au surfaces decreased from 84.9 degrees to 24.8 degrees after the attachment of a highly uniform polydopamine (PDA)/HA hybrid film. The results of surface plasmon resonance analysis showed that the Au-PDA/HA surfaces adsorbed proteins from solutions of bovine serum albumin, lysozyme, beta-lactoglobulin, fibrinogen, and soybean milk in ultralow or low amounts (4.8-31.7 ng/cm(2)). The hydrophilicity and good antifouling performance of the PDA/HA surfaces is attributable to the HA chains that probably attached onto their upper surface via hydrogen bonding between PDA and HA. At the same time, the electrostatic repulsion between PDA and HA probably prevents the aggregation of PDA, resulting in the formation of a highly uniform PDA/HA hybrid film with the HA chains (with a stretched structure) on the upper surface. We also developed a simple method for removing this PDA/HA film and recycling the Au substrates by using an aqueous solution of NaOH as the hydrolyzing agent. The Au surface remained undamaged, and a PDA/HA film could be redeposited on the surface, with the surface exhibiting good antifouling performance even after 10 such cycles. Finally, it was found that this grafting method is applicable to other substrates, including epoxy resins, polystyrene, glass, and steel, owing to the strong adhesion of PDA with these substrates.