Decreased expression of glutathione S-transferase pi correlates with poorly differentiated grade in patients with oral squamous cell carcinoma.
Decreased expression of glutathione S-transferase pi correlates with poorly differentiated grade in patients with oral squamous cell carcinoma.
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DOI：
10.1111/jop.12229
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发表时间：
2015-03
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影响因子：
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通讯作者：
Hailong Ma;Cong Yu;Y. Liu;Yi-ran Tan;Jin-ke Qiao;Xi Yang;Li-Zhen Wang;Jiang Li;Qiong Chen;Fuxiang Chen;Zhi-Yuan Zhang;L. Zhong
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文献类型：
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作者：
Hailong Ma;Cong Yu;Y. Liu;Yi-ran Tan;Jin-ke Qiao;Xi Yang;Li-Zhen Wang;Jiang Li;Qiong Chen;Fuxiang Chen;Zhi-Yuan Zhang;L. Zhong
背景谷胱甘肽S转移酶p1(GSTP1)是第二相解毒酶中的一员，是细胞对应激、缺氧、生长因子等刺激信号的主要调节因子。GSTP1在癌症中的临床作用尚不清楚。本研究旨在探讨口腔鳞状细胞癌(OSCC)患者血清GSTP1水平及口腔鳞癌及OSCC细胞系中GSTP1的表达。方法采用基质辅助激光解吸电离飞行时间质谱仪(MALDITOFMS)对166例口腔鳞癌患者和12 0例正常人进行血清多肽标志物的筛选。用双抗体夹心ELISA法检测18例口腔鳞癌患者和18例正常人血清GSTP1浓度。采用免疫组织化学方法检测28例口腔鳞癌组织标本中GSTP1的表达。采用Western印迹和实时荧光定量聚合酶链式反应检测9株口腔鳞癌组织中GSTP1的表达。结果MALDI-TOF-MS检测发现口腔鳞癌患者血清GSTP1值明显低于正常人(P=0.019)。口腔鳞癌GSTP1mRNA水平和蛋白表达也明显降低。口腔鳞癌组织中GSTP1mRNA的表达与组织分化程度呈正相关，低表达提示口腔鳞癌组织分化程度较差(P=0.041)。结论口腔鳞癌组织中GSTP1表达降低，提示口腔鳞癌组织中GSTP1表达降低，提示口腔鳞癌组织分化程度较差。
BACKGROUND Glutathione S transferase pi (GSTP1) is a member of phase II detoxification enzymes as a major regulator of cell signaling in response to stress, hypoxia, growth factors, and other stimuli. The clinical role of GSTP1 in cancer is still unclear. The aim of this study was to investigate the serum GSTP1 level in patients with oral squamous cell carcinoma (OSCC) and the GSTP1 expression in tissue samples from patients with OSCC and OSCC lines. METHODS One hundred and sixty-six patients with OSCC and 120 normal persons were used to screen potential serum peptide biomarkers using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Serum GSTP1 concentration was detected in 18 patients with OSCC and 18 normal persons using ELISA. Immunohistochemistry was used to detect GSTP1 expression in tissue samples from twenty-eight OSCC patients. Western blot and real-time PCR were used to detect GSTP1 expression in nine OSCC lines. RESULTS Decreased GSTP1 concentration was found in the patients with OSCC compared with the normal persons by MALDI-TOF-MS, which was then confirmed by ELISA (P = 0.019). Decreased GSTP1 mRNA level and protein expression were also found in the OSCC lines. Decreased GSTP1 expression was found correlating with pathological differentiation grade in the tissue samples from OSCC patients, a lower GSTP1 expression indicating a poorer pathological differentiation grade (P = 0.041). CONCLUSIONS These results suggest that decreased GSTP1 expression in patients with OSCC and a lower GSTP1 expression indicating a poorer pathological differentiation grade in OSCC tissue samples.