Myogenin expression in facial muscle following damage to the facial nerve
Myogenin expression in facial muscle following damage to the facial nerve
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DOI：
10.3109/00016489.2011.653671
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发表时间：
2012-06
影响因子：
1.4
通讯作者：
M. Teraoka;N. Hato;Hirotaka Takahashi;Hayato Komobuchi;Naoki Sawai;Masahiro Okada;N. Hakuba
中科院分区：
文献类型：
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作者：
M. Teraoka;N. Hato;Hirotaka Takahashi;Hayato Komobuchi;Naoki Sawai;Masahiro Okada;N. Hakuba
摘要结论：面部肌肉的基因分析可能是检测面部肌肉去神经支配的一种有前途的方法，有助于确定面部麻痹早期进展的预后。目的：在颞内型面瘫的治疗中，神经损伤的早期诊断对于决定治疗方式非常重要。在这项研究中，我们研究了面瘫的严重程度与面部肌肉中表达的肌生成素水平之间的关系。方法：根据面神经是否被切除或压缩，将动物分为两组。使用实时 PCR 和神经损伤后面部肌肉的原位杂交检查肌细胞生成素 mRNA 的表达。结果：神经切除组肌细胞生成素表达增加，而神经压迫组肌细胞生成素表达未见增加。原位杂交表明肌细胞生成素仅在去神经肌纤维周围的卫星细胞中表达。
Abstract Conclusion: Gene analysis of facial muscle may be a promising way to detect denervation of facial muscle, helping to determine the prognosis of a facial palsy early in its progression. Objectives: In the treatment of intratemporal facial palsy, early diagnosis of neural damage is important in deciding about therapeutic modalities. In this study, we investigated the relationship between the severity of facial palsy and the level of myogenin expressed in the facial muscle. Methods: The animals were divided into two groups, depending on whether the facial nerve was resected or compressed. Expression of myogenin mRNAwas examined using real-time PCR and in situ hybridization of the facial muscle following the nerve damage. Results: Increased expression of myogenin was observed in the nerve resection group, while no such increase was seen in the nerve compression group. In situ hybridization indicated that myogenin was expressed exclusively in satellite cells around the denervated muscle fibers.