Evaluation of the dehydration-rehydration method for production of contrast-carrying liposomes.
Evaluation of the dehydration-rehydration method for production of contrast-carrying liposomes.
复制标题
评估用于生产携带造影剂的脂质体的脱水-再水合方法。
DOI：
10.1097/00004424-198802000-00009
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发表时间：
1988
影响因子：
6.7
通讯作者：
Dick,R
中科院分区：
文献类型：
--
作者：
Seltzer,SE;Gregoriadis,G;Dick,R
我们测量了三种类型的放射造影剂（RCM），可以被截留在脂质体制备的脱水-再水化囊泡（DRV）技术的量。为了制备DRV，首先制备含水的小单层囊泡，加入造影剂并冻干混合物。再水化后，DRV重新形成，被动捕获RCM。证明泛影葡胺、碘海醇和碘曲兰的包封量相似（泛影葡胺最好），但所有这些量均低于其他小分子，如羧基荧光素（P< 0.05）。包埋率与脂质浓度成正比（r= 0.76; P< 0.002），与碘浓度成反比（r=-0.86; P< 0.002）。在中性脂质的理想条件下，包封了19.45+/-9.9%的泛影葡胺，相当于1.05+/-0.50 g I/g脂质。这些值接近于大单层囊泡可实现的值。使用自动混合装置（Microfluidizer（R））代替超声处理，促进了大脂质体批次的生产并改善了包封（P< 0.05）。大鼠的计算机断层扫描（CT）扫描显示，每g I/kg注射的DRV分别增强了30和218 HU的肝脏和脾脏。这些研究表明，这种方法（可能由Microfluidizer（R）增强）可以有效地生产造影剂携带脂质体。
We measured the amounts of three types of radiographic contrast media (RCM) that could be entrapped in liposomes prepared by the dehydration-rehydration vesicle (DRV) technique. To make DRVs, one initially makes water-containing, small unilamellar vesicles, adds contrast media and lyophilizes the mixture. Upon rehydration, the DRVs re-form, passively entrapping RCM. Diatrizoate, iohexol and iotrolan proved to be entrappable in similar amounts (diatrizoate was best), but all of these amounts were less than for other small molecules, such as carboxyfluorescein (P< 0.05). Entrapment was directly proportional to lipid concentration (r= 0.76; P< 0.002), and inversely related to iodine concentration (r=-0.86; P< 0.002). Under ideal conditions with neutral lipids, 19.45+/-9.9% of diatrizoate was entrapped, corresponding to 1.05+/-0.50 g I per g lipid. These values are close to those achievable for large unilamellar vesicles. Use of an automated mixing device (the Microfluidizer (R)) in place of sonication, facilitated production of large liposome batches and improved entrapment (P< 0.05) Computed tomography (CT) scans of rats showed 30 and 218 HU of liver and spleen enhancement, respectively, per g I/kg injected DRVs. These studies showed this method (possibly augmented by the Microfluidizer (R)) allows efficient production of contrastcarrying liposomes.