Abrogation of reaginic antibodies with modified allergens
Abrogation of reaginic antibodies with modified allergens
复制标题
用改良的过敏原废除反应抗体
作者：
W. Y. Lee;A. Sehon
花粉热、外源性哮喘、药物过敏和某些形式的荨麻疹等过敏性疾病是由IgE类过敏原特异性抗体(也称为Reagins)介导的。最近，通过注射半抗原与非免疫原性载体1-5或适当修饰的过敏原6-8的结合物，特异性地抑制了针对半抗原和蛋白质抗原的IgE抗体。在这里，我们描述了一种新的方法，将明确定义的蛋白质抗原，如卵蛋白(OA)，或豚草花粉水提物(RAG)的非透析性致敏成分的混合物，转化为能够消除B3D2F1小鼠和大鼠对相应天然变应原的初级和持续的IgE反应的耐受性和非致敏性产品。我们的方法涉及过敏原与非免疫原性、亲水性聚合物聚乙二醇(PEG)的共价偶联；这种方法的灵感来自于有报道称，聚乙二醇与过氧化氢酶和牛血清白蛋白偶联会导致这些蛋白的免疫原性丧失9。
ALLERGIC diseases such as hay fever, extrinsic asthma, drug hypersensitivities and some forms of urticaria are mediated by allergen-specific antibodies of the IgE class, known also as reagins. Specific suppression of the IgE antibodies to haptens and protein antigens has been achieved recently by injection of conjugates of haptens with non-immunogenic carriers1–5 or by appropriately modified allergens6–8. Here we describe a new method for converting a well defined protein antigen, such as ovalbumin (OA), or the mixture of the non-dialysable allergenic constituents of the aqueous extract of ragweed pollen (RAG), to tolerogenic and non-allergenic products which are capable of abrogating the primary as well as ongoing IgE responses to the corresponding native allergens in B3D2F1 mice and rats. Our method involves the covalent coupling of the allergens to the non-immunogenic, hydrophilic polymer, polyethylene glycol (PEG); this approach was inspired by the report that coupling of PEG to catalase and bovine serum albumin resulted in loss of the immunogenicity of these proteins9.