Mutation Rate Inferred From Synonymous Substitutions in a Long-Term Evolution Experiment With Escherichia coli.
Mutation Rate Inferred From Synonymous Substitutions in a Long-Term Evolution Experiment With Escherichia coli.
复制标题
DOI：
10.1534/g3.111.000406
复制
发表时间：
2011-08-01
期刊：
影响因子：
--
通讯作者：
Schneider D
中科院分区：
文献类型：
--
作者：
Wielgoss S;Barrick JE;Tenaillon O;Cruveiller S;Chane-Woon-Ming B;Médigue C;Lenski RE;Schneider D
自发突变率的量化对于从机制上理解进化过程至关重要。在细菌中，使用实验或比较遗传学方法的传统估算容易出现统计上的不确定性，因此估算值的差异超过一个数量级。随着新一代测序技术的出现，现在有可能进行更准确的估算。我们对来自一个40000代进化实验的19个大肠杆菌基因组进行了测序，并基于同义替换的积累直接推断出点突变率。所得估算值为每代每碱基对8.9×10⁻¹¹，并且存在明显偏向于增加的A/T含量。我们还将我们的结果与其他细菌进化实验已发表的基因组序列数据集进行了比较。鉴于我们方法的效力，我们的估算代表了迄今为止可获得的细菌碱基替换率的最准确测量值。
The quantification of spontaneous mutation rates is crucial for a mechanistic understanding of the evolutionary process. In bacteria, traditional estimates using experimental or comparative genetic methods are prone to statistical uncertainty and consequently estimates vary by over one order of magnitude. With the advent of next-generation sequencing, more accurate estimates are now possible. We sequenced 19 Escherichia coli genomes from a 40,000-generation evolution experiment and directly inferred the point-mutation rate based on the accumulation of synonymous substitutions. The resulting estimate was 8.9 × 10−11 per base-pair per generation, and there was a significant bias toward increased AT-content. We also compared our results with published genome sequence datasets for other bacterial evolution experiments. Given the power of our approach, our estimate represents the most accurate measure of bacterial base-substitution rates available to date.