Detection of resistance in Sclerotinia sclerotiorum to carbendazim and dimethachlon in Jiangsu Province of China
Detection of resistance in Sclerotinia sclerotiorum to carbendazim and dimethachlon in Jiangsu Province of China
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DOI：
10.1007/s13313-014-0271-1
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发表时间：
2014-01-01
影响因子：
1.4
通讯作者：
Zhou, Ming-Guo
中科院分区：
文献类型：
--
作者：
Wang, Yong;Hou, Yi-Ping;Zhou, Ming-Guo
菌核菌茎腐病是由菌核菌引起的江苏油菜主要真菌病害，造成油质下降，产量损失10% ~ 80%。化学防治，如多菌灵（MBC）和二甲沙龙，仍然是减少菌丝病发病率的主要方法。为了确定江苏省菌核菌种群对两种杀菌剂的抗性水平，研究了2010 - 2012年从江苏省油籽作物菌核菌感染的茎上采集的分离菌对多菌灵和二甲氯胺的敏感性。药敏试验结果表明，3701株菌株中有375株对多菌灵耐药，耐药频率为10.1%；2010年江苏连云港地区仅有1株菌株在5 μ g/ml二甲基氯浓度下生长正常。江苏不同地区多菌灵耐药菌株比例为0 ~ 44%。田间抗药菌株的菌丝生长、菌核产量、致病性和渗透敏感性与田间敏感菌株相当，表明田间抗药菌株可能具有足够的寄生适应度与田间敏感菌株竞争；而抗二甲沙龙的分离株在上述方面不如亲本分离株适合。此外，菌核菌对二甲沙龙与异丙酮、原嘧米酮等其他二甲肟类杀菌剂表现出正交叉抗性。
Sclerotinia stem rot caused by Sclerotinia sclerotiorum, is the main fungal disease of oilseed rape (Brassica napus) in Jiangsu Province of China, causing oil quality declines and yield losses from 10 to 80 %. Chemical control, such as carbendazim (MBC) and dimethachlon, remains the major method to reduce the incidence of S. sclerotiorum. Isolates collected from Sclerotinia-infected stems of oilseed crops in Jiangsu province between 2010 and 2012, were tested for their sensitivity to carbendazim and dimethachlon in order to determine the level of resistance to these fungicides in the population of S. sclerotiorum in that province. Sensitivity tests showed that 375 isolates were resistant to carbendazim among the 3,701 isolates with a resistance frequency of 10.1 %; only one isolate in the Lian Yungang region of Jiangsu Province in 2010 showed normal growth at 5 mu g/ml dimethachlon. The proportion of carbendazim-resistant isolates ranged from 0 to 44 % in different regions of Jiangsu. The field MBC-resistant isolates showed comparable mycelial growth, sclerotial production, pathogenicity and osmotic sensitivity to the MBC-sensitive isolates, which suggested that the field MBC-resistant isolates might have sufficient parasitic fitness to compete with the field sensitive isolates in the field; whereas the dimethachlon-resistant isolates were less fit than their parental isolates in terms above. Moreover, S. sclerotiorum exhibited positive cross-resistance between dimethachlon and other dicarboximide fungicides such as iprodione and procymidone.