A comparison of stress tolerance in YPD and industrial lignocellulose-based medium among industrial and laboratory yeast strains
A comparison of stress tolerance in YPD and industrial lignocellulose-based medium among industrial and laboratory yeast strains
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DOI：
10.1007/s10295-009-0592-1
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发表时间：
2009-08-01
影响因子：
3.4
通讯作者：
Larsson, Christer
中科院分区：
文献类型：
--
作者：
Albers, Eva;Larsson, Christer
一般来说，人们认为酵母在恶劣的工业条件下发酵，特别是在使用木材水解液或木质纤维底物等底物的情况下，需要使用所谓的工业菌株。为了验证这是否总是正确的，使用两个工业菌株和四个常用的实验室菌株，CEN-PK和X2180的单倍体和二倍体版本，在工业相关的压力条件下进行了性能比较。这些工业菌株是一种瑞典商业面包师酵母菌株，以及一种以前从使用木质纤维素底物的工业生物乙醇生产厂分离出来的菌株。胁迫条件包括，除了在木质纤维底物本身生长外，葡萄糖、氯化钠、乙醇和乳酸的浓度升高，以及低pH。结果表明，在该类型的培养基中，适应木质纤维基质的菌株也具有最高的生长速度和最短的滞后期。然而，与其他应变相比，附加应力水平越高，差异越小，尤其是X2180表现出对这些附加应力条件的高抵抗力。此外，在实验室菌株的单倍体和二倍体版本之间没有检测到性能上的差异。也许，至少在某些情况下，像X2180这样的实验室菌株可能是一种具有工业吸引力的生产有机体，其优势是促进了进行受控基因操作的可能性。
In general, it is believed that fermentation by yeast under harsh industrial conditions, especially if substrates such as wood hydrolysate or lignocellulosic substrates are used, requires the use of so-called industrial strains. In order to check whether this is always true, a comparison of performance was made using two industrial strains and four commonly used laboratory strains, the haploid and diploid versions of CEN-PK and X2180, under industrially relevant stress conditions. The industrial strains were a Swedish commercial baker's yeast strain and a strain previously isolated from an industrial bioethanol production plant using lignocellulosic substrate. Stress conditions included, apart from growth in the lignocellulosic substrate itself, elevated concentrations of glucose, NaCl, ethanol, and lactate as well as low pH. Results showed that, indeed, the strain adapted to lignocellulosic substrate also possessed the highest growth rate as well as shortest duration of the lag phase in this type of medium. However, the higher the additional stress level, the lower the difference compared to other strains, and X2180 in particular displayed a high resistance to these additional stress conditions. Furthermore, no difference in performance could be detected between the haploid or diploid versions of the laboratory strains. It might be that, at least under some circumstances, a laboratory strain such as X2180 could be an industrially attractive production organism with the advantage of facilitating the possibilities for making controlled genetic manipulations.