Chitotriosidase activity in patients with interstitial lung diseases
Chitotriosidase activity in patients with interstitial lung diseases
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DOI：
10.1016/j.rmed.2007.05.008
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发表时间：
2007-10-01
影响因子：
4.3
通讯作者：
Rottoli, Paola
中科院分区：
文献类型：
--
作者：
Bargagli, Elena;Margollicci, Maria;Rottoli, Paola
背景：在之前的论文中，我们发现结节病患者的血清和 BAL 中壳三糖苷酶（一种巨噬细胞衍生酶）的活性显着高于对照组，尤其是在疾病进展和肺部受累的患者中。局部和全身产生的壳三糖苷酶活性与放射学分期以及肺浸润程度相关，提示该酶可能在结节病的发病机制中发挥作用，并可作为疾病严重程度的标志物。方法：通过荧光测定法测定壳三糖苷酶活性。结果：结果表明，血清壳三糖苷酶活性仅在结节病患者中升高；而结节病患者中血清壳三糖苷酶活性仅在结节病患者中升高。在特发性肺纤维化和与系统性硬化症相关的肺纤维化患者中，该值处于正常范围。相反，结节病和特发性肺纤维化患者的BAL中其活性显着高于对照组。结论：血清壳三糖苷酶是结节病严重程度的潜在标志物；结节病的发病率与放射学分期和肺部浸润程度相关。结节病和特发性肺纤维化患者 BAL 中壳三糖苷酶活性的增加表明该酶可能参与弥漫性肺病的纤维形成。需要进一步的研究来了解壳三糖苷酶在结节病发病机制中的作用及其在某些弥漫性肺病纤维化重塑中的作用。 (C) 2007 Elsevier Ltd. 保留所有权利。
Background: In previous papers, we found significantly higher activity of chitotriosidase, a macrophage derived enzyme, in serum and BAL of patients with sarcoidosis, especially in those with progressing disease and lung involvement, than in controls. Locally and systemically produced chitotriosidase activity was correlated with radiological stage and also with degree of lung infiltration, suggesting that this enzyme may play a rote in the pathogenesis of sarcoidosis and may be used as a marker of disease severity.Aim: To analyse chitotriosidase activity in serum and bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis and pulmonary fibrosis associated with systemic sclerosis and to compare it with chitotriosidase activity in controls and sarcoidosis patients.Methods: Chitotriosidase activity was determined by a fluorometric assay.Results: The results showed that serum chitotriosidase activity was only elevated in sarcoidosis patients; in patients with idiopathic pulmonary fibrosis and pulmonary fibrosis associated with systemic sclerosis it was in the normal range. On the contrary, in BAL of sarcoidosis and idiopathic pulmonary fibrosis patients the activity was significantly higher than in controls.Conclusion: Serum chitotriosidase is a potential marker of sarcoidosis severity; it increases in sarcoidosis in relation to radiological stage and degree of lung infiltration. The increase in chitotriosidase activity in BAL of sarcoidosis and idiopathic pulmonary fibrosis patients suggests that the enzyme could be involved in fibrogenesis in diffuse lung diseases. Further research is needed to understand the role of chitotriosidase in the pathogenesis of sarcoidosis and its involvement in fibrotic remodelling in certain diffuse lung diseases. (C) 2007 Elsevier Ltd. All rights reserved.