Antibiotic resistance, antimicrobial residues and bacterial community composition in urban wastewater
Antibiotic resistance, antimicrobial residues and bacterial community composition in urban wastewater
复制标题
DOI：
10.1016/j.watres.2013.01.010
复制
发表时间：
2013-04-01
期刊：
影响因子：
12.8
通讯作者：
Manaia, Celia M.
中科院分区：
文献类型：
--
作者：
Novo, Ana;Andre, Sandra;Manaia, Celia M.
本研究基于以下假设：污水中抗生素残留和抗生素耐药菌的发生可能与最终出水的细菌群落结构和组成以及抗生素耐药性负荷相关。从一个城市污水处理厂收集了超过14个采样日期的原始和处理后的废水复合样品。对样本进行了以下表征：i）四环素类、青霉素类、磺胺类、喹诺酮类、三氯生、砷、镉、铅、铬和汞的出现率; ii）四环素类、磺胺甲恶唑、环丙沙星和阿莫西林的抗生素耐药百分比; iii）16 S rRNA基因-DGGE图谱。相应的样品的数据，考虑到水力停留时间，进行了分析，使用多变量analysis.Variations上的最终出水的细菌群落结构的变化显着相关的四环素类，青霉素类，磺胺类，喹诺酮类和三氯生在原流入的发生。Epsilonproteobacteria类的成员与这些抗菌剂呈现正相关，而β和γ-proteobacteria和厚壁菌门观察到负相关。抗生素耐药百分比呈现不同的变化趋势，异养菌/肠杆菌和肠球菌，随时间和污水处理后。抗生素耐药性与四环素类药物残留和高温呈正相关。抗生素残留，细菌群落结构和组成和抗生素耐药性之间的关系被证明。涉及更多废水处理厂的进一步研究可能有助于阐明这种复杂的关系。(C)2013爱思唯尔有限公司保留所有权利。
This study was based on the hypothesis that the occurrence of antimicrobial residues and antibiotic resistant bacteria in the sewage could be correlated with the structure and composition of the bacterial community and the antibiotic resistance loads of the final effluent. Raw and treated wastewater composite samples were collected from an urban treatment plant over 14 sampling dates. Samples were characterized for the i) occurrence of tetracyclines, penicillins, sulfonamides, quinolones, triclosan, arsenic, cadmium, lead, chromium and mercury; ii) antibiotic resistance percentages for tetracycline, sulfamethoxazole, ciprofloxacin and amoxicillin and iii) 16S rRNA gene-DGGE patterns. The data of corresponding samples, taking into account the hydraulic residence time, was analyzed using multivariate analysis.Variations on the bacterial community structure of the final effluent were significantly correlated with the occurrence of tetracyclines, penicillins, sulfonamides, quinolones and triclosan in the raw inflow. Members of the class Epsilonproteobacteria presented positive correlations with those antimicrobials, whereas negative correlations were observed with Beta and Gammaproteobacteria and Firmicutes. Antibiotic resistance percentages presented different trends of variation in heterotrophs/enterobacteria and in enterococci, varied over time and after wastewater treatment. Antibiotic resistance was positively correlated with the occurrence of tetracyclines residues and high temperature. A relationship between antibiotic residues, bacterial community structure and composition and antibiotic resistance is demonstrated. Further studies, involving more wastewater treatment plants may help to elucidate this complex relationship. (C) 2013 Elsevier Ltd. All rights reserved.