THE N-TERMINAL HYDROPHOBIC REGION OF THE MATURE PHOSPHATE TRANSLOCATOR IS SUFFICIENT FOR TARGETING TO THE CHLOROPLAST INNER ENVELOPE MEMBRANE
THE N-TERMINAL HYDROPHOBIC REGION OF THE MATURE PHOSPHATE TRANSLOCATOR IS SUFFICIENT FOR TARGETING TO THE CHLOROPLAST INNER ENVELOPE MEMBRANE
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DOI：
10.1105/tpc.7.9.1421
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发表时间：
1995-09-01
期刊：
影响因子：
11.6
通讯作者：
GRAY, JC
中科院分区：
文献类型：
--
作者：
KNIGHT, JS;GRAY, JC
为了定位所需的序列，指导磷酸转运到叶绿体内被膜，一系列的嵌合蛋白组成部分的磷酸转运和小亚基的核酮糖-1，5-二磷酸羧化酶/加氧酶，这是通常位于基质中，已经产生。磷酸转运蛋白和小亚基的前序列和成熟序列的相互交换表明磷酸转运蛋白前序列包含基质靶向信息，并且成熟蛋白负责内包膜膜靶向。嵌合蛋白含有N-末端46个氨基酸残基的磷酸转运蛋白被定向到内包膜膜。该区域细分成其复合亲水性和疏水性区域表明，仅由氨基酸残基24至45组成的疏水性区域能够将核酮糖-1，5-二磷酸羧化酶/加氧酶的小亚基引导至内被膜。
To locate the sequence required for directing the phosphate translocator to the chloroplast inner envelope membrane, a series of chimeric proteins constituting parts of the phosphate translocator and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, which is normally located in the stroma, has been produced. Reciprocal exchanges of the presequences and mature sequences of the phosphate translocator and the small subunit indicated that the phosphate translocator presequence contains stromal targeting information and that the mature protein is responsible for inner envelope membrane targeting. Chimeric proteins containing the N-terminal 46 amino acid residues of the phosphate translocator were directed to the inner envelope membrane. Subdivision of this region into its composite hydrophilic and hydrophobic regions showed that the hydrophobic region alone, which consists of amino acid residues 24 to 45, was able to direct the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase to the inner envelope membrane.