Involvement of the Central Nervous System in the Salivary Secretion Induced by Pilocarpine in Rats
Involvement of the Central Nervous System in the Salivary Secretion Induced by Pilocarpine in Rats
复制标题
毛果芸香碱诱导大鼠唾液分泌中枢神经系统的参与
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
J. Menani
中科院分区：
文献类型：
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作者：
A. Renzi;E. Colombari;T. R. Mattos;J. Silveira;W. A. Saad;L. Camargo;L. A. D. Luca;J. G. Derobio;J. Menani
在大鼠中，前腹侧第三脑室（AV 3V）电解质损伤对腹腔内（i. p.）或脑室内（i. c. v.）研究了注射胆碱能激动剂（匹鲁卡品）。使用用氨基甲酸乙酯麻醉的假手术或AV 3V损伤大鼠，并将不锈钢套管植入侧脑室（LV）。在静脉注射或腹腔注射毛果芸香碱后7分钟内研究唾液分泌量。在假手术大鼠中，腹腔注射毛果芸香碱（1 mg/kg b.w.）（6 h、2、7和15天后）产生唾液分泌（分别为486 ± 21、778 ± 85、630 ± 50和560 ± 55 mg/7 min）。在AV 3V损伤后6小时、2天和7天，该效应降低（分别为142 ± 22、113 ± 32和290 ± 62 mg/7 min），但在AV 3V损伤后15天未降低（516 ± 19 mg/7 min）。在假手术大鼠中，I. c. v.注射毛果芸香碱（120 μg/1 μL）后6 h、2、7和15天也产生唾液分泌（分别为443 ± 20、417 ± 81、496 ± 14和427 ± 47 mg/7 min）。在AV 3V损伤后6小时、2天和7天，i. c. v.毛果芸香碱的作用也降低（分别为143 ± 19、273 ± 14和322 ± 17 mg/7 min），但在15天后没有降低（450 ± 28 mg/7 min）。结果表明，中枢神经系统，特别是AV 3V区域，是重要的毛果芸香碱对大鼠唾液分泌的影响。提示中枢通路的激活可能在大鼠唾液向外周毛果芸香碱的分泌中起重要作用。
The effect in rats of an anteroventral third ventricle (AV3V) electrolytic lesion on salivary secretion induced by intraperitoneal (i.p.) or intracerebroventricular (i.c.v.) injection of a cholinergic agonist (pilocarpine) was investigated. Sham- or AV3V-lesioned rats anesthetized with urethane and with a stainless steel cannula implanted into the lateral ventricle (LV) were used. The amount of salivary secretion was studied over a seven-minute period after i.c.v. or i.p. injection of pilocarpine. In sham-operated rats, i.p. injection of pilocarpine (1 mg/kg b.w.) (after 6 h, 2, 7, and 15 days) produced salivary secretion (486 ± 21, 778 ± 85, 630 ± 50, and 560 ± 55 mg/7 min, respectively). This effect was reduced 6 h, 2, and 7 days after an AV3V lesion (142 ± 22, 113 ± 32, and 290 ± 62 mg/7 min, respectively), but not 15 days after an AV3V lesion (516 ± 19 mg/7 min). I.c.v. injection of pilocarpine (120 μg in 1 μL), in sham-operated rats after 6 h, 2, 7, and 15 days also produced salivary secretion (443 ± 20, 417 ± 81, 496 ± 14, and 427 ± 47 mg/7 min, respectively). The effects of i.c.v. pilocarpine were also reduced 6 h, 2, and 7 days after an AV3V lesion (143 ± 19, 273 ± 14, and 322 ± 17 mg/7 min, respectively), but not after 15 days (450 ± 28 mg/7 min). The results demonstrate that the central nervous system, and particularly the AV3V region, is important for the effect of pilocarpine on salivary secretion in rats. Moreover, they suggest that activation of central pathways may play an important part in the salivary secretion to peripheral pilocarpine in rats.