Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair
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DOI：
10.1126/science.1101598
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发表时间：
2004-12-03
期刊：
影响因子：
56.9
通讯作者：
Essen, LO
中科院分区：
文献类型：
--
作者：
Mees, A;Klar, T;Essen, LO
DNA光解酶利用光能修复含有紫外线诱导损伤的DNA，如顺式同步环丁烷嘧啶二聚体（CPDs）。在这里，我们报告了DNA光解酶结合的晶体结构，双链DNA弯曲50度，包括一个合成的CPD损伤。在100 K的同步辐射下，CPD病变被翻转到活性部位，并在那里分裂成两个胸腺嘧啶。虽然光解酶仅在200k以上催化蓝光驱动的CPD切割，但这种结构显然模仿了光驱动DNA修复过程中的结构亚状态，其中胸腺嘧啶的反向翻转尚未发生成双工DNA。
DNA photolyases use light energy to repair DNA that comprises ultrarviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place.