Saccharomyces cerevisiae RNA polymerase I terminates transcription at the Reb1 terminator in vivo.
Saccharomyces cerevisiae RNA polymerase I terminates transcription at the Reb1 terminator in vivo.
复制标题
酿酒酵母 RNA 聚合酶 I 在体内终止 Reb1 终止子处的转录。
DOI：
10.1128/mcb.19.11.7369
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发表时间：
1999
影响因子：
5.3
通讯作者：
Roan,JG
中科院分区：
文献类型：
--
作者：
Reeder,RH;Guevara,P;Roan,JG
我们绘制了酵母中RNA聚合酶I的转录终止位点。S1酶切图谱分析表明，主要终止子是位于25SrRNA 3′端下游+93位的Reb1p终止子。反转录结合定量PCR显示，大约90%的所有转录本终止于该位点。通过+93位点读取的转录物定量地终止于位于更下游+250处的故障安全终止子。Rnt1p（一种参与加工25S rRNA 3′端的RNase III）的失活极大地稳定了延伸到两个位点的转录本，并增加了+93位点的通读。Reb1p终止子突变体的体内测定表明，该位点在体内通过与先前通过体外研究描绘的相同机制起作用。
We have mapped transcription termination sites for RNA polymerase I in the yeastSaccharomyces cerevisiae. S1 nuclease mapping shows that the primary terminator is the Reb1p terminator located at +93 downstream of the 3′ end of 25S rRNA. Reverse transcription coupled with quantitative PCR shows that approximately 90% of all transcripts terminate at this site. Transcripts which read through the +93 site quantitatively terminate at a fail-safe terminator located further downstream at +250. Inactivation of Rnt1p (an RNase III involved in processing the 3′ end of 25S rRNA) greatly stabilizes transcripts extending to both sites and increases readthrough at the +93 site. In vivo assay of mutants of the Reb1p terminator shows that this site operates in vivo by the same mechanism as has previously been delineated through in vitro studies.