A bipolar kinesin
A bipolar kinesin
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DOI：
10.1038/379270a0
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发表时间：
1996-01-18
期刊：
影响因子：
64.8
通讯作者：
Scholey, JM
中科院分区：
文献类型：
--
作者：
Kashina, AS;Baskin, RJ;Scholey, JM
有丝分裂过程中的染色体分离依赖于有丝分裂纺锤体的作用，有丝分裂纺锤体是一种自组织的双极性蛋白质机器，它使用微管（mt）及其相关的马达（1,2）。运动蛋白相关mt -马达蛋白的BimC亚家族成员被认为对正常双极纺锤体的形成和功能至关重要（3-14）。在这里，我们报道了KRP(130)，一种从黑腹果蝇胚胎中纯化的同源四聚体bimc相关蛋白（13），具有超微结构。它由四个与肌球蛋白相关的多肽组装成两极聚集体，两端有运动结构域，类似于微型肌球蛋白丝（15）。这种双极“微丝”可以使纺锤体mt交联，并使它们相对滑动。我们不知道任何其他MT电机具有双极结构。
CHROMOSOME Segregation during mitosis depends on the action of the mitotic spindle, a self-organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors(1,2). Members of the BimC subfamily of kinesin-related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle(3-14). Here we report that KRP(130), a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos(13), has an ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament(15). Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.