Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato
Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato
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DOI：
10.1016/s2095-3119(16)61553-8
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发表时间：
2017-01-01
影响因子：
4.8
通讯作者：
Jian Heng
中科院分区：
文献类型：
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作者：
Jin Na;Xue Hui;Jian Heng
红灰链霉菌HDZ-9-47，分离自根结线虫属的卵，在离体条件下和保护地条件下，评价了其作为南方根结线虫生防药剂的潜力。显微观察表明，HDZ-9-47寄生于黑腹叶蜂卵内。7天内匿名。HDZ-9-47的培养滤液在离体条件下对中华绒螯蟹二龄幼虫的致死率为97.0%。对卵孵化有50%以上的抑制作用。田间试验结果表明，与对照相比，接种后90d，灌服含1012个HDZ-9-47孢子的发酵液处理的根结指数和J2 s密度分别降低了51.1%和80.7%，优于其他施用剂量和方法。此外，HDZ-9-47与生物熏蒸联合处理的根结指数和J2 s密度降低率分别为87.1%和91.0%，优于HDZ-9-47、生物熏蒸和噻唑膦单独处理。番茄产量也增加了16.1%。以上结果表明，HDZ-9-47可能是一种有效的生防菌。HDZ-9-47与甘蓝渣生物熏蒸联合应用是一种有前途和可持续的选择。匿名控制
Streptomyces rubrogriseus HDZ-9-47, isolated from eggs of Meloidogyne spp., was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective field. Microscopic observations showed that HDZ-9-47 parasitized eggs of M. incognita within 7 days. In vitro, the culture filtrate of HDZ-9-47 caused 97.0% mortality of second-stage juveniles (J2s) of M. incognita and inhibited more than 50% egg hatching. In the field, compared with the control, the root knot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7% at 90 days post transplantation, which were better than that in other application doses and methods. In addition, reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%, respectively, better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment. And tomato yield also increased by 16.1%. Together, our results suggest that HDZ-9-47 could be an effective biocontrol agent of M. incognita, and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M. incognita control.