Viral metagenomics revealed novel betatorquevirus species in pediatric inpatients with encephalitis/meningoencephalitis from Ghana
Viral metagenomics revealed novel betatorquevirus species in pediatric inpatients with encephalitis/meningoencephalitis from Ghana
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DOI：
10.1038/s41598-019-38975-z
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发表时间：
2019-02-20
影响因子：
4.6
通讯作者：
Cadar, Daniel
中科院分区：
文献类型：
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作者：
Eibach, Daniel;Hogan, Benedikt;Cadar, Daniel
尽管对大量病原体进行了广泛的调查，但儿科患者急性脑炎/脑膜脑炎的原因仍然无法解释。为了探讨可能的病毒的影响，我们调查了70例临床兼容的脑炎/脑膜脑炎发热儿科住院患者的脑脊液标本的病毒组。使用病毒宏基因组学，我们检测和遗传特征的三个新的人类转矩teno迷你病毒（TTMV）的物种（TTMV-G1-3）。系统发生学上，TTMV-G1-3聚集在三个新的单系属倍他克病毒的Anelloviridae家庭。TTMV-G1-3在患病儿童中高度流行，但在健康队列中不存在，这可能表明TTMV种类与发热性疾病相关。由于2/3检测到疟疾合并感染，尚不清楚这些新的环节病毒属是否是病原体或通过与疟疾寄生虫相互作用而增加疾病的严重程度。开始抗疟和/或抗生素治疗后28天病毒的存在表明病毒感染仍然活跃，可能是寄生虫和/或细菌合并感染的影响，这可能启动了病毒复制的免疫系统环境调节或病毒清除缺陷。这项研究增加了目前对TTMV遗传多样性的认识，并加强了人环状病毒可以被认为是某些病理条件下免疫系统强烈扰动的生物标志物。
The cause of acute encephalitis/meningoencephalitis in pediatric patients remains often unexplained despite extensive investigations for large panel of pathogens. To explore a possible viral implication, we investigated the virome of cerebrospinal fluid specimens of 70 febrile pediatric inpatients with clinical compatible encephalitis/meningoencephalitis. Using viral metagenomics, we detected and genetically characterized three novel human Torque teno mini virus (TTMV) species (TTMV-G1-3). Phylogenetically, TTMV-G1-3 clustered in three novel monophyletic lineages within genus Betatorquevirus of the Anelloviridae family. TTMV-G1-3 were highly prevalent in diseased children, but absent in the healthy cohort which may indicate an association of TTMV species with febrile illness. With 2/3 detected malaria co-infection, it remains unclear if these novel anellovirus species are causative agents or increase disease severity by interaction with malaria parasites. The presence of the viruses 28 days after initiating antimalarial and/or antibiotic treatment suggests a still active viral infection likely as effect of parasitic and/or bacterial co-infection that may have initiated a modulated immune system environment for viral replication or a defective virus clearance. This study increases the current knowledge on the genetic diversity of TTMV and strengthens that human anelloviruses can be considered as biomarkers for strong perturbations of the immune system in certain pathological conditions.