Characterization of a Potential Ripening Regulator, SlNAC3, from Solanum Lycopersicum.
Characterization of a Potential Ripening Regulator, SlNAC3, from Solanum Lycopersicum.
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DOI：
10.1515/biol-2018-0062
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发表时间：
2018-01
影响因子：
2.2
通讯作者：
Han Q
中科院分区：
文献类型：
--
作者：
Jing L;Li J;Song Y;Zhang J;Chen Q;Han Q
NAC（NAM、ATAF 1 -2和CUC 2）蛋白是植物中最大的转录因子家族之一。它们具有多种功能，与果实的发育过程密切相关。番茄是研究果实生长规律的模式植物。本研究对一种新的NAC类转录因子SlNAC 3（SGN-U 568609）的功能特性和作用机制进行了研究，以确定其在番茄发育和成熟中的作用。原核表达SlNAC 3蛋白，免疫新西兰白色兔，获得特异性多克隆抗体。通过免疫共沉淀和MALDI-TOF-MS分析，我们发现SlNAC 3蛋白和多聚半乳糖醛酸酶-2（PG-2）之间存在相互作用，这与果实的成熟和软化有关。染色质免疫沉淀测定揭示了高度胁迫耐受性的潘那利番茄染色体10（序列ID，HG975449.1）的基因组，进一步证明了SlNAC 3是番茄中干旱和盐胁迫抗性的负调节因子，与先前的报道一致。这些结果表明，SlNAC 3不仅参与非生物胁迫，而且在介导番茄成熟中起着必要的作用。
NAC (for NAM, ATAF1-2, and CUC2) proteins are one of the largest transcription factor families in plants. They have various functions and are closely related to developmental processes of fruits. Tomato (Solanum lycopersicum) is a model plant for studies of fruit growth patterns. In this study, the functional characteristics and action mechanisms of a new NAC-type transcription factor, SlNAC3 (SGN-U568609), were examined to determine its role in tomato development and ripening. The SlNAC3 protein was produced by prokaryotic expression and used to immunize New Zealand white rabbits to obtain a specific polyclonal antibody against SlNAC3. By co-immunoprecipitation and MALDI-TOF-MS assays, we showed that there was an interaction between the SlNAC3 protein and Polygalacturonase-2 (PG-2), which is related to the ripening and softening of fruit. Chromatin immunoprecipitation assays revealed the genome of the highly stress-tolerant Solanum pennellii chromosome 10 (sequence ID, HG975449.1), further demonstrating that SlNAC3 is a negative regulator of drought and salinity stress resistance in tomato, consistent with previous reports. These results indicate that SlNAC3 is not only involved in abiotic stress, but also plays a necessary role in mediating tomato ripening.