Inhibition of proliferation and induction of apoptosis by EGCG in human osteogenic sarcoma (HOS) cells
Inhibition of proliferation and induction of apoptosis by EGCG in human osteogenic sarcoma (HOS) cells
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DOI：
10.1007/bf02968585
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发表时间：
2006-05-01
影响因子：
6.7
通讯作者：
Kim, Jeong Hee
中科院分区：
文献类型：
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作者：
Ji, Sang Jin;Han, Dong Hoon;Kim, Jeong Hee
EGCG [(-)-表没食子儿茶素-3-盖酸酯]是绿茶的主要成分，被认为是主要的抗氧化剂成分。除了被认为作为化学预防剂和化疗剂用于癌症治疗之外，EGCG 最近还被认为具有抗增殖作用。我们重新审视了本研究中的后一个发现，并特别关注 EGCG 诱导人成骨肉瘤 (HOS) 细胞凋亡的能力。根据形态变化、染色体 DNA 降解和亚 G 凋亡细胞群的增加，EGCG 的抗增殖作用（IC50 = 35.3 +/- 6.0 mu g/mL）似乎与凋亡细胞死亡有关。用 EGCG 处理 HOS 细胞逐渐激活 caspase-3，这是一种已确定的细胞凋亡诱导剂。
EGCG [(-)-epigallocatechin-3-gailate], a major component of green tea has been considered as a major antioxidant constituent. In addition to having been considered for cancer treatment as a chemopreventive and chemotherapeutic agent, EGCG has recently been attributed an anti-proliferative effect. We re-examined the latter finding in this study and added specific focus on the ability of EGCG to induce apoptosis in human osteogenic sarcoma (HOS) cells. Antiproliferative action of EGCG (IC50 = 35.3 +/- 6.0 mu g/mL) appeared to be linked to apoptotic cell death based on morphological changes, chromosomal DNA degradation, and an increase in the sub-G, apoptotic cell population. Treatment of HOS cells with EGCG gradually activated caspase-3, an established inducer of apoptotic cell death.