Changes in the inner and outer retinal layers after acute increase of the intraocular pressure in adult albino Swiss mice
Changes in the inner and outer retinal layers after acute increase of the intraocular pressure in adult albino Swiss mice
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DOI：
10.1016/j.exer.2010.05.020
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发表时间：
2010-08-01
影响因子：
3.4
通讯作者：
Vidal-Sanz, Manuel
中科院分区：
文献类型：
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作者：
Cuenca, Nicolas;Pinilla, Isabel;Vidal-Sanz, Manuel
在成年白化病小鼠中，每隔3-14周研究眼压升高对外视网膜及其回路的影响。最近有报道称，通过烧灼小鼠眼睛前部的血管可诱发眼高压（OHT） （Salinas-Navarro等，2009a）。同时记录双眼视网膜电图（ERG）的反应，并在激光照射前和激光照射后2周、8周和12周的不同生存期进行比较。在激光照射后3、9或14周对动物进行处理，在低温恒温器中获得径向切片，并使用抗recoverin、γ -转导素、蛋白激酶c - α (pkc - α)、calbindin或synaptophysin的抗体进一步进行免疫细胞化学处理。突触带用抗蛋白质巴松管的抗体鉴定，标记光感受器带和细胞核用TO-PRO鉴定。激光光凝左眼的鞘周静脉和锁骨上静脉导致平均眼压在24小时内增加到大约两倍于基线，并维持了大约5天，在1周后达到基础水平。不同组小鼠的ERG记录显示，在激光照射12周后，与对侧眼相比，它们的a波、b波和暗位阈值反应（STR）幅度分别降低至62%、52%和23%。激光照射三周后，用恢复素和转导素抗体进行免疫染色，外核层（ONL）未见任何变化，但ON-rod双极细胞和水平细胞外丛状层（OPL）的树突状突起均已消失。激光照射14周后，观察到ONL中水平和双极细胞过程的芽出，蛋白激酶- c抗体显示ON-rod双极细胞的形态改变，calbindin染色显示水平细胞异常，其与突触前输入的关系丧失。此外，在这个时候，定量研究表明，与其他眼睛相比，光感受器突触带的数量/100 μ m显著减少，外核和丛状层的厚度也显著减少。瑞士小鼠眼压升高导致其全视野ERG反应的永久性改变以及视网膜内外回路的改变。(C) 2010 Elsevier Ltd.版权所有。
In adult albino mice the effects of increased intraocular pressure on the outer retina and its circuitry was investigated at intervals ranging 3-14 weeks. Ocular hypertension (OHT) was induced by cauterizing the vessels draining the anterior part of the mice eye, as recently reported (Salinas-Navarro et al., 2009a). Electroretinographic (ERG) responses were recorded simultaneously from both eyes and compared each other prior to and at different survival intervals of 2,8 or 12 weeks after lasering. Animals were processed at 3, 9 or 14 weeks after lasering, and radial sections were obtained in the cryostat and further processed for immunocytochemistry using antibodies against recoverin, gamma-transducin, Protein Kinase C-alpha (PKC-alpha), calbindin or synaptophysin. The synaptic ribbons were identified using an antibody against the protein bassoon, which labels photoreceptor ribbons and nuclei were identified using TO-PRO. Laser photocoagulation of the perilimbar and episcleral veins of the left eye resulted in an increase in mean intraocular pressure to approximately over twice its baseline by 24 h that was maintained for approximately five days reaching basal levels by 1 week. ERG recordings from the different groups of mice showed their a-, b-wave and scotopic threshold response (STR) amplitudes, when compared to their contralateral fellow eye, reduced to 62%, 52% and 23% at 12 weeks after lasering.Three weeks after lasering, immunostaining with recoverin and transducin antibodies could not document any changes in the outer nuclear layer (ONL) but both ON-rod bipolar and horizontal cells had lost their dendritic processes in the outer plexiform layer (OPL). Sprouting of horizontal and bipolar cell processes were observed into the ONL Fourteen weeks after lasering, protein kinase-C antibodies showed morphologic changes of ON-rod bipolar cells and calbindin staining showed abnormal horizontal cells and a loss of their relationship with their presynaptic input. Moreover, at this time, quantitative studies indicate significant diminutions in the number of photoreceptor synaptic ribbons/100 mu m, and in the thickness of the outer nuclear and plexiform layer, when compared to their fellow eyes. Increased intraocular pressure in Swiss mice results in permanent alterations of their full field ERG responses and in changes of the inner and outer retinal circuitries. (C) 2010 Elsevier Ltd. All rights reserved.