Effect of potassium on the renin-angiotensin system and HgCl 2 -induced acute renal failure.
Effect of potassium on the renin-angiotensin system and HgCl 2 -induced acute renal failure.
复制标题
钾对肾素-血管紧张素系统和HgCl 2 诱导的急性肾衰竭的影响。
DOI：
10.1152/ajplegacy.1973.224.2.305
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
J. S. McNeil
中科院分区：
文献类型：
--
作者：
W. Flamenbaum;T. Kotchen;R. Nagle;J. S. McNeil
方法给体重在185和250 g之间的雄性Sprague-Dawley大鼠1喂食含有0.287 mEq钠/g和0.207 mEq钾/g的基础饮食（GL Baking Co.，Frederick，Md.）和自来水。在指示的情况下，用2%氯化钾溶液或1%氯化钠溶液代替自来水。在设计用于分离尿液和粪便的单个代谢笼中平衡2天后，在对照大鼠中测定24小时平衡，如下所示：测量液体和食物摄入量，计算钠和钾的消耗量;测量尿量和尿渗透压摩尔浓度，测定钠和钾排泄量。在用2%氯化钾替代自来水后1、2或3周，在钾负荷大鼠中进行了类似的24小时平衡研究。对照组和钾负荷组动物用戊巴比妥钠（50 mg/ml）（50 mg/kg）腹腔内麻醉，以在24小时平衡研究后立即测定菊粉清除率和血浆体积。对两条股静脉进行导管插入，并通过腹侧切口将凸缘导管（PE-90）置于膀胱中，并用荷包缝合线固定。对先前描述的方法进行了修改，用于测定血浆体积（37）。简言之，使用微米注射器静脉内给予精确体积的稀释在0.85%盐水中的1251标记的人血清白蛋白（Albumutope，ER Squibb & Sons，新玩法，NJ），并用0.2ml盐水冲洗导管。通过向计数管中递送至少4个相同体积，测定给药剂量的总放射性。个别样品的范围小于3%。注射后10分钟，抽取0.4 ml血液。在进行本报告中描述的研究时，研究者遵守由国家科学院-国家研究理事会实验动物资源研究所实验动物设施和护理指南委员会颁布的实验动物设施和护理指南。
METHODSMale Sprague-Dawley rats1 weighing between 185 and 250 g were fed a basic diet containing 0.287 mEq sodium per gram and 0.207 mEq potassium per gram (GL Baking Co., Frederick, Md.) and tap water. Where indicated, either a 2% potassium chloride solution or a 1% sodium chloride solution was substituted for tap water. After 2 days of equilibration in individual metabolic cages designed to separate urine from feces, a 24-hr balance was determined in control rats as follows: fluid and food intake were measured, and the amounts of sodium and potassium consumed were calculated; urine volume and urine osmolality were measured, and sodium and potassium excretion were determined. Similar 24-hr balance studies were obtained in potassium-loaded rats 1, 2, or 3 weeks after substitution of 2% potassium chloride for tap water. Control and potassium-loaded animals were anesthetized with sodium pentobarbital(50 mg/ml), 50 mg/kg intraperitoneally, for the determination of inulin clearance and plasma volume immediately after the 24-hr balance studies. Both femoral veins were catheterized and a flanged catheter (PE-90) was placed in the bladder through a ventral incision and secured with a purse-string suture. A modification of a previously described method was used for the determination of plasma volumes (37). Briefly, a precise volume of 1251-labeled human serum albumin(Albumutope, ER Squibb & Sons, New Brunswick, NJ) diluted in 0.85% saline was given intravenously using a micrometer syringe, and the catheter was flushed with 0.2 ml of saline. The total radioactivity of the administered dose was determined by delivering at least 4 identical volumes into counting tubes. The range of individual samples was less than 3%. Ten minutes after the injection, 0.4 ml of blood was withdrawn l In conducting the research described in this report, the investigators adhered to the Guide for Laboratory Animal Facilities and Care, as promulgated by the Committee on the Guide for Laboratory Animal Facilities and Care of the Institute of Laboratory Animal Resources, National Academy of Sciences-National Research Council.