Lipidomics revealed the global lipid responses of primary bovine alveolar macrophages to infections of Mycobacterium tuberculosis and Mycobacterium bovis
Lipidomics revealed the global lipid responses of primary bovine alveolar macrophages to infections of Mycobacterium tuberculosis and Mycobacterium bovis
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脂质组学揭示了原代牛肺泡巨噬细胞对结核分枝杆菌和牛分枝杆菌感染的整体脂质反应。
DOI：
10.1016/j.intimp.2021.108407
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发表时间：
2022-02-01
影响因子：
5.6
通讯作者：
Wang, Yujiong
中科院分区：
文献类型：
--
作者：
Gao, Weifeng;Cai, Yurong;Wang, Yujiong
结核分枝杆菌（M.结核分枝杆菌（MTB）和M.结核分枝杆菌复合体（MTBC）的牛（MB）是包括牛和人在内的一系列哺乳动物中臭名昭著的传染病结核（TB）的病原体。MTB/MB的脂质成分在入侵宿主巨噬细胞时发挥着重要作用。然而，MTB和MB的脂质组成的详细变化是未知的，而MTB/MB感染宿主的脂质代谢相关的反应也不清楚。本研究采用双脂质组学方法研究MTB和MB脂质组成的差异以及MTB/MB感染原代牛肺泡巨噬细胞后脂质代谢的变化。脂质组学显示这两种分枝杆菌属之间的脂质组成存在显著差异，特别是甘油磷脂、甾醇脂质、脂肪酰和聚酮化合物水平的差异。同时，MTB和MB均可引起宿主巨噬细胞的多种脂质代谢反应。结核分枝杆菌感染主要引起巨噬细胞聚酮化合物和甘油磷脂的增加，而MB感染则引起甘油磷脂和甾醇的增加。此外，MTB和PC的TAG 13：0-18：5-18：5（16：1（9 E）/0：0）、PI（20：2（11 Z，14 Z）/22：6（4 Z，7Z，10 Z，13 Z，16 Z，19 Z））、4，6-癸二炔-1-醇异戊酸酯和MB的LacCer（d18：1/24：1（15 Z））分别被鉴定为引起巨噬细胞脂质代谢的变化。从这些数据中，我们提出了MTB和MB中的脂质成分的差异组成可以通过不同的机制成功地在巨噬细胞中定植。MTB可促进巨噬细胞泡沫细胞的形成，以利于其定植和发育，而MB主要抑制巨噬细胞的自噬，以逃避宿主的免疫反应。
Mycobacterium tuberculosis (M. tuberculosis (MTB) and M. bovis (MB) of the Mycobacterium tuberculosis complex (MTBC) are the causative agents of the notorious infectious disease tuberculosis (TB) in a range of mammals, including bovine and human. The lipid composition of MTB/MB performed imperative function as invading host macrophage. However, the detailed variations in lipid compositions of MTB and MB were unknown, while the responses relevant to lipid metabolisms in MTB/MB-infected host were also unclear. In the present study, a dualLipidomics were used to elucidate the differences in lipid composition of MTB and MB and responses in lipid metabolisms of primary bovine alveolar macrophages infected by MTB/MB. The Lipidomics showed significant differences in lipid composition, especially differences in levels of Glycerophospholipids, Sterol Lipids, Fatty Acyls and Polyketides between these two mycobacterium species. Meanwhile, both MTB and MB could invoke various responses of lipid metabolisms in host macrophages. An infection of MTB mainly induced the increases of Polyketides and Glycerophospholipids in macrophages, whereas an MB infection induced the increases of Glycerophospholipids and Sterol. Furthermore, TAG 13:0-18:5-18:5 of MTB and PC (16:1(9E)/0:0), PI(20:2 (11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), 4, 6-Decadiyn-1-ol isovalerate and LacCer (d18:1/24:1(15Z)) of MB were identified to cause variations in lipid metabolisms of macrophages, respectively. From these data, we proposed that the differential compositions of lipid compositions in MTB and MB could successfully colonize in macrophage by different mechanisms. MTB could promote the formation of foam cells of macrophage for its colonization and development, while MB mainly suppresses the macrophage autophagy to escape the immune responses of host.