Silkworm plasmatocytes are more resistant than other hemocyte morphotypes to Bombyx mori nucleopolyhedrovirus infection
Silkworm plasmatocytes are more resistant than other hemocyte morphotypes to Bombyx mori nucleopolyhedrovirus infection
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DOI：
10.1016/j.jip.2012.09.004
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发表时间：
2013-01-01
影响因子：
3.4
通讯作者：
Katsuma, Susumu
中科院分区：
文献类型：
--
作者：
Hori, Takanori;Kiuchi, Takashi;Katsuma, Susumu
多种昆虫血细胞形态的病毒易感性差异至今尚未被研究。本研究利用含有果蝇hsp70启动子驱动绿色荧光蛋白（GFP）基因的家蚕核多角体病毒（BmNPV）衍生物，观察了mari B.幼虫血细胞的NPV趋向性。实验清楚地显示，当通过腹腔内或口服途径感染时，gfp阳性的浆细胞少于其他类型的血细胞，如粒细胞、类粒细胞和球细胞。我们的研究结果表明，家蚕浆细胞比其他类型的血细胞更能抵抗BmNPV感染。(C) 2012爱思唯尔公司版权所有。
Differences in the viral susceptibility of multiple insect hemocyte morphotypes have not been investigated to date. In this study, a Bombyx mori nucleopolyhedrovirus (BmNPV) derivative possessing a Drosophila hsp70 promoter-driven green fluorescent protein (GFP) gene was used to observe NPV tropism of B. mari larval hemocytes. The experiments clearly revealed that there were fewer GFP-positive plasmatocytes than those observed in other types of hemocytes, such as granulocytes, oenocytoids, and spherulocytes, when infected via the intrahemocoelic or oral route. Our results indicate that silkworm plasmatocytes are more resistant than other hemocyte morphotypes to BmNPV infection. (C) 2012 Elsevier Inc. All rights reserved.