Transcriptome analyses provide insights into the effect of temperature change on fiber quality of ramie
Transcriptome analyses provide insights into the effect of temperature change on fiber quality of ramie
复制标题
转录组分析有助于深入了解温度变化对苎麻纤维质量的影响
DOI：
10.1016/j.indcrop.2020.112544
复制
发表时间：
2020-09-15
影响因子：
5.9
通讯作者：
Peng, Dingxiang
中科院分区：
文献类型：
--
作者：
Guo, Pingan;Wang, Bo;Peng, Dingxiang
季节性温度变化导致苎麻、亚麻等麻类作物纤维品质的差异，但在RNA水平上对温度变化在纤维发育中的调控作用的认识还很有限。本研究采用转录组测序技术，在模拟不同收获季节温度条件下，探讨环境温度对不同发育程度苎麻纤维品质的影响。我们从两个全长转录组中分别获得了73 335和66 195个单基因。基于比较转录组学分析，我们共鉴定出14 228个差异表达基因，其中一些基因与细胞壁组装或生物发生、细胞膨胀、纤维素合成等有关。我们发现，温度变化影响纤维细胞大小和原料纤维化学成分的合成，高温处理延迟了苎麻树皮的木质化进程，并鉴定了5个与木质化进程密切相关的MYB转录因子。本研究结果为探索温度变化下纤维品质形成的机理提供了宝贵的资源，为纤维品质的遗传改良奠定了基础。
Seasonal temperature change contributes to differential fiber quality in bast fiber crops such as ramie, flax, etc. However, the knowledge of the regulatory role of temperature change in fiber development at the RNA level is limited. Here, we used transcriptome sequencing to explore how ambient temperature affects the quality of ramie fiber with different developmental degrees under simulated temperature conditions for different harvest seasons. We obtained 73 335 and 66 195 unigenes from two full-length transcriptomes, respectively. Based on comparative transcriptomics analysis, we identified a total of 14 228 differentially expressed genes, some of which are related to cell wall assembly or biogenesis, cell expansion, and cellulose synthesis, etc. We found that temperature change influenced fiber cell size and the synthesis of chemical constituents of the raw fiber and, specifically high temperature delayed the lignification of ramie bark and identified five MYB transcription factors closely related to the lignification process. Our findings provide a valuable resource for exploring the mechanism of fiber quality formation under temperature change and lay the foundation for genetic improvement of fiber quality.