Comparison of tamarins and marmosets as hosts for GBV-B infections and the effect of immunosuppression on duration of viremia
Comparison of tamarins and marmosets as hosts for GBV-B infections and the effect of immunosuppression on duration of viremia
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DOI：
10.1016/s0042-6822(03)00193-4
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发表时间：
2003-06-20
期刊：
影响因子：
3.7
通讯作者：
Brasky, KM
中科院分区：
文献类型：
--
作者：
Lanford, RE;Chavez, D;Brasky, KM
GBV-B病毒是导致绢毛猴肝炎的丙型肝炎病毒（HCV）的近亲，因此是HCV的有吸引力的替代模型。在这项研究中，我们证明了GBV-B的宿主范围延伸到普通的绒猴，其感染情况与观察到的绢毛猴相似。绒猴肝细胞对GBV-B体外感染敏感。病毒被有效分泌到培养基中，大约25%的肝细胞NS 3染色呈阳性。为了诱导持续感染，用FK 506抑制绢毛猴的免疫，并接种GBV-B。尽管未诱导慢性感染，但大多数动物的病毒血症持续时间延长。在1只动物中，病毒血症持续时间延长至46周，但病毒清除发生在停止FK 506治疗后18周。与绢毛猴相比，绒猴的更大可用性将极大地促进未来使用该模型的研究工作。(C)2003 Elsevier Science（美国）。All rights reserved.
GBV-B virus is a close relative to hepatitis C virus (HCV) that causes hepatitis in tamarins, and thus, is an attractive surrogate model for HCV. In this study, we demonstrate that the host range of GBV-B extends to the common marmoset with an infection profile similar to that observed for tamarins. Marmoset hepatocytes were susceptible to in vitro infection with GBV-B. Virus was efficiently secreted into the medium, and approximately 25% of hepatocytes were positive for NS3 staining. In an attempt to induce persistent infections, tamarins were immunosuppressed with FK506 and inoculated with GBV-B. Although no chronic infections were induced, the duration of viremia was increased in most animals. In one animal, the duration of viremia was extended to 46 weeks, but viral clearance occurred 18 weeks after stopping FK506 therapy. The greater availability of marmosets in comparison to tamarins will greatly facilitate future research efforts with this model. (C) 2003 Elsevier Science (USA). All rights reserved.