Limited chloroplast gene transfer via recombination overcomes plastomegenome incompatibility between Nicotiana tabacum and Solanum tuberosum
Limited chloroplast gene transfer via recombination overcomes plastomegenome incompatibility between Nicotiana tabacum and Solanum tuberosum
复制标题
DOI：
10.1007/bf00017450
复制
发表时间：
2004
影响因子：
5.1
通讯作者：
N. D. Thanh;P. Medgyesy
中科院分区：
文献类型：
--
作者：
N. D. Thanh;P. Medgyesy
在亚种间烟草-茄组合中，原生质体融合后不能选择新的核-叶绿体组合的绿色杂种。作为一种克服质体基因组不相容的方法，部分质体转移已被考虑通过基因重组。将一种光敏烟草质体突变体原生质体与辐照致死的野生马铃薯原生质体融合后，在2.5×104colonies中恢复了一个单一的绿色菌落。再生植株具有类似烟草的形态（虽然不正常），但通常呈绿色，对腱毒素敏感，表现出马铃薯亲本的叶绿体标记。叶绿体DNA （cpDNA）的限制性内切酶分析显示重组，非亲代模式。与亲本cpDNA物理图谱的比较表明，存在相当一部分马铃薯质体，两侧是烟草特异性区域。这种“烟草”质体在回交和回融合实验中被证明是稳定的，并且在仅存在氮的情况下正常发挥作用。tabacumnucleus。
Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilialNicotiana-Solanumcombination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of protoplasts of a light-sensitiveNicotiana tabacum(tobacco) plastome mutant and lethally irradiated protoplasts of wild-typeSolanum tuberosum(potato), a single green colony was recovered among 2.5×104colonies. The regenerated plants had tobacco-like (although abnormal) morphology, but were normally green, and sensitive to tentoxin, demonstrating chloroplast markers of the potato parent. Restriction enzyme analysis of the chloroplast DNA (cpDNA) revealed recombinant, nonparental patterns. A comparison with physical maps of the parental cpDNA demonstrated the presence of a considerable part of the potato plastome flanked by tobacco-specific regions. This “potacco” plastome proved to be stable in backcross and backfusion experiments, and normally functional in the presence solely ofN. tabacumnucleus.