Differential mitogenic effects of methyl isobutyl xanthine and a tumor growth factor on G1-arrested 3T3 T proadipocytes at the predifferentiation GD state and the growth-factor deficiency GS state.
Differential mitogenic effects of methyl isobutyl xanthine and a tumor growth factor on G1-arrested 3T3 T proadipocytes at the predifferentiation GD state and the growth-factor deficiency GS state.
复制标题
甲基异丁基黄嘌呤和肿瘤生长因子对处于预分化 GD 状态和生长因子缺乏 GS 状态的 G1 停滞的 3T3 T 前脂肪细胞的不同促有丝分裂作用。
DOI：
10.1016/0014-4827(83)90067-8
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发表时间：
1983
影响因子：
3.7
通讯作者：
Florine,DL
中科院分区：
文献类型：
--
作者：
Scott,RE;Yun,K;Florine,DL
在3 T3 T前脂肪细胞周期的G1期存在两种生长状态。GD是前脂肪细胞在分化之前必须生长停滞的停滞状态。GSI是前脂肪细胞在血清或生长因子剥夺后生长停滞的停滞状态。在试图进一步区分这些逮捕状态，我们比较了各种有丝分裂原诱导GD和GS逮捕的细胞启动DNA合成的相对能力。结果表明，甲基异丁基黄嘌呤（MIX）能诱导高密度和低密度GD-阻滞细胞增殖，而高密度和低密度GS-阻滞细胞则不能。数据还显示，肿瘤生长因子可以刺激高密度和低密度GD-和GS-停滞细胞的增殖，而其他试剂对于高密度GD-停滞细胞是不良的有丝分裂原。我们的结论是，MIX和肿瘤生长因子（TUGF）可以作为探针来研究GDarrest状态的特征。
Two growth-states exist in the G1 phase of the 3T3 T proadipocyte cell cycle. GDis the arrest state at which proadipocytes must growth-arrest prior to differentiation. GSis the arrest state at which proadipocytes growth-arrest following deprivation of serum or growth factors. In an attempt to further distinguish these arrest states, we have compared the relative ability of a variety of mitogens to induce GD- and GS-arrested cells to initiate DNA synthesis. The data show that GD-arrested cells at both high and low densities can be induced to proliferate by methyl-isobutyl-xanthine (MIX), whereas high and low density GS-arrested cells are not. The data also show that a tumor growth factor can stimulate the proliferation of both high and low density GD- and GS-arrested cells, whereas other agents are poor mitogens for high density GD-arrested cells. We conclude that MIX and a tumor growth factor (TUGF) can serve as probes to study the characteristics of the GDarrest state.