Deletion of the carboxy-terminus of herpes simplex virus type 1 (HSV-1) glycoprotein B does not affect oligomerization, heparin-binding activity, or its ability to protect against HSV challenge.
Deletion of the carboxy-terminus of herpes simplex virus type 1 (HSV-1) glycoprotein B does not affect oligomerization, heparin-binding activity, or its ability to protect against HSV challenge.
复制标题
删除 1 型单纯疱疹病毒 (HSV-1) 糖蛋白 B 的羧基末端不会影响寡聚化、肝素结合活性或其抵御 HSV 攻击的能力。
DOI：
10.1007/bf01718618
复制
发表时间：
1996
影响因子：
2.7
通讯作者：
Cantin,EM
中科院分区：
文献类型：
--
作者：
Lin,XH;Ali,MA;Openshaw,H;Cantin,EM
A recombinant vaccinia virus designated VgBt which expresses a truncated secreted herpes simplex virus gB (gBt) was constructed and compared to V11gB, a vaccinia recombinant previously studied which expresses gB exclusively on the surface of infected cells. Indirect immunofluorescence assay (IFA) revealed that gBt was strongly associated with the surface of infected cells despite being released slowly into the cell culture medium. Both gB and gBt existed as oligomers, and both membrane bound and secreted forms of gBt exhibited heparin-binding activity. In protection studies VgBt and V11gB conferred equivalent protection against both homologous (HSV-1) and heterologous (HSV-2) challenge with HSV.
