PATTERNS OF CELL-DIVISION REVEALED BY TRANSCRIPTIONAL REGULATION OF GENES DURING THE CELL-CYCLE IN PLANTS
PATTERNS OF CELL-DIVISION REVEALED BY TRANSCRIPTIONAL REGULATION OF GENES DURING THE CELL-CYCLE IN PLANTS
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DOI：
10.1002/j.1460-2075.1994.tb06299.x
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发表时间：
1994-02-01
期刊：
影响因子：
11.4
通讯作者：
DOONAN, JH
中科院分区：
文献类型：
--
作者：
FOBERT, PR;COEN, ES;DOONAN, JH
来自五个细胞周期相关基因的转录本在分离的细胞中积累，分散在植物分生组织活跃的分裂区域。我们认为这种模式反映了细胞分裂周期特定阶段的基因表达。分离细胞的高比例表明，子代细胞之间的同步性在有丝分裂后迅速丧失。这是第一次在高等生物体中描述这种细胞特异性表达模式。DNA特异性染料的复染表明，三个基因(两个有丝分裂周期蛋白和一个类似cdc2的基因)的转录本在间期和早期有丝分裂的部分期间积累，而组蛋白H4基因的转录本只优先在间期细胞中检测到。细胞周期蛋白和组蛋白H4转录本的双重标记证实，这些基因在不同的细胞中表达，因此在细胞周期的不同阶段表达。结果表明，细胞周期相关基因的转录调控可能在控制植物细胞分裂中起重要作用，这些基因是识别植物分生组织中处于细胞周期特定阶段的细胞的有用标记。
Transcripts from five cell cycle related genes accumulate in isolated cells dispersed throughout the actively dividing regions of plant meristems. We propose that this pattern reflects gene expression during particular phases of the cell division cycle. The high proportion of isolated cells suggests that synchrony between daughter cells is rapidly lost following mitosis. This is the first time that such a cell specific expression pattern has been described in a higher organism. Counterstaining with a DNA specific dye revealed that transcripts from three genes (two mitotic cyclins and a cdc2-like gene) accumulate during part of interphase and early mitosis whereas transcripts from a histone H4 gene are preferentially detected only in interphase cells. Double labelling for cyclin and histone H4 transcripts confirms that these genes are expressed in different cells, and therefore at different phases of the cell cycle. The results suggest that transcriptional regulation of cell cycle related genes may be important in controlling cell division in plants, and that these genes are useful markers for identifying cells at specific phases of the cell cycle within plant meristems.