Oral administration of the milk casein-derived tripeptide Val-Pro-Pro attenuates high-fat diet-induced adipose tissue inflammation in mice
Oral administration of the milk casein-derived tripeptide Val-Pro-Pro attenuates high-fat diet-induced adipose tissue inflammation in mice
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DOI：
10.1017/s0007114514001147
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发表时间：
2014-08-28
影响因子：
3.6
通讯作者：
Yoshida, Masayuki
中科院分区：
文献类型：
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作者：
Aihara, Kotaro;Osaka, Mizuko;Yoshida, Masayuki
脂肪组织的炎症引发代谢综合征、动脉粥样硬化和CHD。在本研究中，我们研究了牛奶酪蛋白衍生的三肽缬氨酸-脯氨酸-脯氨酸（VPP）是否对高脂饮食（HFD）喂养的小鼠的脂肪组织具有抗炎作用。雄性C57 BL/6 J小鼠（7周龄）自由采食HFD和普通自来水（HFD组）或HFD和含有0.3mg VPP/ml的水（HFD+VPP组）10周。结果显示，与HFD组相比，HFD+VPP组外周血单核细胞中CD 18的表达水平显著降低。活化的单核细胞和促炎性巨噬细胞在HFD喂养小鼠的脂肪组织的基质血管部分中积累，在补充VPP的小鼠中显著减少。在给予VPP的小鼠的脂肪组织中，富含促炎性巨噬细胞的冠状结构的形成也显著减少。实时PCR分析显示，单核细胞趋化蛋白-1和促炎细胞因子IL-6在脂肪组织中的表达在HFD+VPP组中倾向于低于HFD组。这些观察结果表明，口服给予VPP对HFD喂养小鼠的脂肪组织产生抗炎作用，这可能最终导致慢性炎症相关疾病的一级预防。
Inflammation of adipose tissue triggers the metabolic syndrome, atherosclerosis and CHD. In the present study, we investigated whether the milk casein-derived tripeptide valine-proline-proline (VPP) has an anti-inflammatory effect on the adipose tissue of high-fat diet (HFD)fed mice. Male C57BL/6J mice (7 weeks of age) were fed ad libitum with either a HFD and plain tap water (HFD group) or a HFD and water containing 0.3mg VPP/ml (HFD+VPP group) for 10 weeks. The results showed that the expression level of CD18 in the peripheral blood monocytes of the HFD+VPP group was significantly decreased compared with the level observed in those of the HFD group. Activated monocytes and pro-inflammatory macrophages were accumulated in the stromal vascular fractions of the adipose tissue from HFD-fed mice, which were significantly decreased in those supplemented with VPP. The formation of crown-like structures rich in pro-inflammatory macrophages was also significantly reduced in the adipose tissue of mice administered with VPP. Real-time PCR analysis revealed that the expression of monocyte chemoattractant protein-1 and that of the pro-inflammatory cytokine IL-6 in adipose tissue tend to be lower in the HFD+VPP group than in the HFD group. These observations indicate that oral administration of VPP exerts an anti-inflammatory effect on the adipose tissue of HFD-fed mice, which may eventually lead to the primary prevention of chronic inflammation-related diseases.