Gene expression profiling of endobronchial ultrasound (EBUS)-derived cytological fine needle aspirates from hilar and mediastinal lymph nodes in non-small cell lung cancer.
Gene expression profiling of endobronchial ultrasound (EBUS)-derived cytological fine needle aspirates from hilar and mediastinal lymph nodes in non-small cell lung cancer.
复制标题
非小细胞肺癌肺门和纵隔淋巴结支气管内超声（EBUS）衍生细胞学细针抽吸物的基因表达谱。
DOI：
10.1111/cyt.12034
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Lee R
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文献类型：
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作者：
Lee R
R.Lee，D.J.Cousins，E.Ortiz-Zapater，R.Breen，E.McLean和G.Santis非小细胞肺癌患者经纤维支气管镜(EBUS)取自肺门和纵隔淋巴结的细胞学细针抽吸物的基因表达谱研究目的：经支气管腔内超声(EBUS)可以对肺门和纵隔淋巴结进行微创采样，在非小细胞肺癌(NSCLC)的诊断和分期中具有重要作用。在肺癌的研究中，分子生物标记物的探索越来越多。基因表达谱(GEP)是一种基于微阵列的技术，它全面评估基因组范围内基因表达的变化，可以为肿瘤特异性分子签名提供预测预后和治疗反应的可能性。我们评估了从良性和肿瘤浸润性淋巴结中提取的EBUS提取液用于GEP的可行性。方法：在常规临床实践中，从EBUS引导下的细针吸取标本中提取RNA。随后对6例NSCLC患者进行了GEP检查，其中3例为肿瘤浸润性结节，3例为良性淋巴结节，比较其基因表达的差异。结果：32例患者中有29例成功提取RNA，其中12例被诊断为NSCLC。扩增GEP后，RNA得率(中位数，12.1RNAg)和RNA完整性(中位数，6.3GEP)是足够的。用主成分分析和层次聚类方法对腺癌的良、恶性结节进行区分。结论：在临床实践中，对已知的非小细胞肺癌患者的良性和肿瘤浸润性淋巴结行EBUS细针抽吸物的RNA提取和GEP是可行的。需要对更大的患者队列进行进一步研究，以确定有力区分非小细胞肺癌良性和恶性淋巴结节的表达谱。
R. Lee, D. J. Cousins, E. Ortiz‐Zapater, R. Breen, E. McLean and G. SantisGene expression profiling of endobronchial ultrasound (EBUS)‐derived cytological fine needle aspirates from hilar and mediastinal lymph nodes in non‐small cell lung cancerObjective:Endobronchial ultrasound (EBUS) allows minimally invasive sampling of hilar and mediastinal lymph nodes and has an established role in non‐small cell lung cancer (NSCLC) diagnosis and staging. Molecular biomarkers are being explored increasingly in lung cancer research. Gene expression profiling (GEP) is a microarray‐based technology that comprehensively assesses genome‐wide changes in gene expression that can provide tumour‐specific molecular signatures with the potential to predict prognosis and treatment responsiveness. We assessed the feasibility of using EBUS‐derived aspirates from benign and tumour‐infiltrated lymph nodes for GEP.Methods:RNA was extracted from EBUS‐directed transbronchial fine needle aspiration samples in routine clinical practice. GEP was subsequently performed in six patients with NSCLC, three of whom had tumour‐infiltrated nodes and three who had benign lymph nodes; the differences in gene expression were then compared.Results:RNA was successfully extracted in 29 of 32 patients, 12 of whom were diagnosed with NSCLC. RNA yield (median, 12.1 μg) and RNA integrity (median, 6.3) were sufficient after amplification for GEP. Benign and malignant nodes in adenocarcinoma were discriminated by principal component analysis and hierarchical clustering with different expression patterns between malignant and benign nodes.Conclusion:We have demonstrated the feasibility of RNA extraction and GEP on EBUS‐derived transbronchial fine needle aspirates from benign and tumour‐infiltrated lymph nodes in patients with known NSCLC in routine clinical practice. Further studies on larger patient cohorts are required to identify expression profiles that robustly differentiate benign from malignant lymph nodes in NSCLC.