Design of novel primer sets for easy detection of Ruegeria species from seawater
Design of novel primer sets for easy detection of Ruegeria species from seawater
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DOI：
10.1080/09168451.2019.1700776
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发表时间：
2019-12-09
影响因子：
1.6
通讯作者：
Kataoka, Michihiko
中科院分区：
文献类型：
--
作者：
Kitamura, Ruriko;Miura, Natsuko;Kataoka, Michihiko
一些与珊瑚有关的细菌显示出保护珊瑚免受病原体侵害的作用。然而，保护珊瑚的细菌在海水中的分布尚不清楚。此外，与研究珊瑚病原体的方法相比，研究保护珊瑚细菌的方法很少。本文制备了一种简便的检测方法，其中一些菌株抑制珊瑚病原菌珊瑚弧菌的生长。我们通过硅筛选和体外筛选成功获得了两组鲁氏杆菌靶向引物。引物组r38F-r30R和r445F-r446R，以及新设计的通用引物组U357 ' F-U515 ' R，使用从大阪收集的海水中提取的环境DNA进行体外评价。这些方法和引物将有助于揭示鲁氏菌属在海洋环境中的分布。
Some coral-associated bacteria show protective roles for corals against pathogens. However, the distribution of coral-protecting bacteria in seawater is not well known. In addition, compared with the methods for investigating coral pathogens, few methods have been developed to detect coral-protecting bacteria. Here we prepared a simple method for detecting Ruegeria spp., some strains of which inhibit growth of the coral pathogen Vibrio coralliilyticus. We successfully obtained two Ruegeria-targeting primer sets through in silico and in vitro screening. The primer sets r38F-r30R and r445F-r446R, in addition to the newly designed universal primer set U357 ' F-U515 ' R, were evaluated in vitro using environmental DNA extracted from seawater collected in Osaka. These methods and primers should contribute to revealing the distribution of Ruegeria spp. in marine environments.