A basolateral chloride conductance in rat lingual epithelium.
A basolateral chloride conductance in rat lingual epithelium.
复制标题
大鼠舌上皮基底外侧氯电导。
DOI：
10.1007/s002329900396
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Mierson,S
中科院分区：
文献类型：
--
作者：
Wladkowski,SL;Lin,W;McPheeters,M;Kinnamon,SC;Mierson,S
我们使用Ussing室测量和全细胞记录来表征大鼠舌上皮的氯离子电导。尼氟酸（NFA）和氟芬那酸（FFA）是一种非甾体抗炎芳香族化合物，已知可抑制其他组织中的Cl−电导，当从丝氨酸侧加入时，可降低完整大鼠舌背前部上皮的跨上皮短路电流（Isc），并降低大鼠真菌状味觉细胞的全细胞电流。在尤辛室和膜片钳实验中，NFA的作用通过用甲磺酸盐或葡萄糖酸盐代替Cl−来模拟。在低Cl−浴溶液中，NFA对全细胞电流的影响降低。用Ba2+代替Ca2+降低了全细胞Cl−电流。我们的结论是，钙激活的Cl−电导可能存在于大鼠舌上皮的基底外侧膜，并存在于菌状乳头的味觉感受器细胞。需要进一步的实验来确定这种传导在味觉传导中的作用。
We used Ussing chamber measurements and whole-cell recordings to characterize a chloride conductance in rat lingual epithelium. Niflumic acid (NFA) and flufenamic acid (FFA), nonsteroidal anti-inflammatory aromatic compounds known to inhibit Cl−conductances in other tissues, reduced transepithelial short-circuit current (Isc) in the intact dorsal anterior rat tongue epithelium when added from the serosal side, and reduced whole-cell currents in rat fungiform taste cells. In both Ussing chamber and patch-clamp experiments, the effect of NFA was mimicked by replacement of bath Cl−with methanesulfonate or gluconate. In low Cl−bath solution, the effect of NFA on whole-cell current was reduced. Replacement of bath Ca2+with Ba2+reduced the whole-cell Cl−current. We conclude that a Ca2+-activated Cl−conductance is likely present in the basolateral membrane of the rat lingual epithelium, and is present in the taste receptor cells from fungiform papillae. Further experiments will be required to identify the role of this conductance in taste transduction.