Bactericidal Efficacy of a Two-Dimensional Array of Integrated, Coaxial, Microhollow, Dielectric Barrier Discharge Plasma Against Salmonella enterica Serovar Heidelberg
Bactericidal Efficacy of a Two-Dimensional Array of Integrated, Coaxial, Microhollow, Dielectric Barrier Discharge Plasma Against Salmonella enterica Serovar Heidelberg
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DOI：
10.1089/fpd.2019.2698
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发表时间：
2019-10-14
影响因子：
2.8
通讯作者：
Goyal, Sagar M.
中科院分区：
文献类型：
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作者：
Aboubakr, Hamada A.;Nisar, Muhammad;Goyal, Sagar M.
我们研究了冷大气压等离子体（CAP），由二维阵列的集成，同轴，微空心，介质阻挡放电等离子体，对肠道沙门氏菌血清型海德堡（SH）不锈钢，生菜，鸡胸肉的功效。在干燥的不锈钢表面上，SH暴露于CAP的杀菌效力较低;暴露10分钟后，仅2.5 log（10）菌落形成单位（CFU）被灭活。另一方面，水分的存在导致去污类似的6.5 log（10）CFU后，只有3分钟。虽然完全去污后，生菜和鸡胸肉样品的曝光10分钟，SH计数分别减少了类似的4.5和3.7 log（10）CFU。在钢表面上观察到杀菌作用的部分抑制，当其在加标细菌和暴露于等离子体之前用牛血清白蛋白涂覆时，表明鸡肉的蛋白质性质可能是CAP对鸡肌肉功效较低的部分原因。还发现初始细菌负荷影响抗SH功效;在高（类似于6.5 log CFU）和低（类似于3.5 CFU）初始计数下，不锈钢和生菜上完全去污所需的时间分别从3分钟减少到0.5分钟和>10分钟减少到1分钟。然而，灭活动力学分析表明，初始污染负荷对细菌灭活率的影响没有统计学意义。这与两种细菌载量均低于可能影响杀灭率的多层阈值的条件下的其他发现一致。
We studied the efficacy of cold atmospheric-pressure plasma (CAP), generated by a two-dimensional array of integrated, coaxial, microhollow, dielectric barrier discharge plasma, against Salmonella enterica serovar Heidelberg (SH) on stainless steel, romaine lettuce, and chicken breast. Exposure of SH to CAP on a dry stainless steel surface had low bactericidal efficacy; only 2.5 log(10) colony-forming units (CFUs) were inactivated after 10 min of exposure. On the other hand, the presence of moisture led to decontamination of similar to 6.5 log(10) CFUs after only 3 min. Although complete decontamination was not achieved on lettuce and chicken breast samples after 10 min of exposure, SH counts were reduced by similar to 4.5 and 3.7 log(10) CFUs, respectively. A partial suppression of bactericidal effects was observed on steel surfaces when it was coated with bovine serum albumin before spiking with bacteria and exposure to plasma, indicating that the proteinaceous nature of chicken meat may be partially responsible for lower efficacy of CAP on chicken muscles. The initial bacterial load was also found to affect the anti-SH efficacy; at high (similar to 6.5 log CFUs) and low (similar to 3.5 CFUs) initial counts, the time required for complete decontamination on stainless steel and lettuce decreased from 3 to 0.5 min and >10 to 1 min, respectively. However, the analysis of inactivation kinetics showed that effects of initial loads of contamination on the rate of bacterial inactivation were not statistically significant. This is consistent with other findings for conditions where both bacterial loads were under the multilayering threshold that might have affected the rate of killing.