Kinetic analysis of apolipoproteins in postprandial hypertriglyceridaemia rabbits
Kinetic analysis of apolipoproteins in postprandial hypertriglyceridaemia rabbits
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DOI：
10.1258/la.2008.007004
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发表时间：
2009-04-01
影响因子：
2.4
通讯作者：
Ohwada, K.
中科院分区：
文献类型：
--
作者：
Hata, M.;Ito, T.;Ohwada, K.
餐后高脂血症（PHT）是一种以餐后高脂血症、中心性肥胖和糖耐量异常为特征的新型代谢综合征动物模型。为探讨PHT家兔的脂代谢特点，测定了血浆载脂蛋白A-Ⅰ、B、C-Ⅱ、C-Ⅲ和E的含量。使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳研究载脂蛋白B100和B48的运动，以确定餐后甘油三酯增加是外源性还是内源性的。PHT家兔饲喂后载脂蛋白A-Ⅰ、B、C-Ⅱ和E水平升高。在d < 1.006 g/mL的PHT兔血浆组分中检测到载脂蛋白B100和B48。PHT家兔中载脂蛋白B的餐后增加反映了血液中脂蛋白颗粒的数量增加;载脂蛋白C-II和E的增加表明脂蛋白催化剂的某些紊乱。餐后检测PHT家兔的载脂蛋白B48。这些结果表明，外源性脂质的延迟catalysis引起的乳糜微粒残留物在血液中的保留。结果还表明，脂肪分解酶脂蛋白脂肪酶和肝甘油三酯脂肪酶的活动是缺乏和外源性脂蛋白的肝摄取延迟PHT兔。特别是在检测人体残余高脂蛋白血症时，PHT兔是一种很好的高脂蛋白血症研究动物模型。
The postprandial hypertriglyceridaemia (PHT) rabbit, developed as a new animal model of metabolic syndrome, is characterized by PHT, central obesity and glucose intolerance. For detailed investigation of lipid metabolism characteristics in PHT rabbit, the plasma levels of apolipoproteins A-I, B, C-II C-III and E were measured. Movements of apolipoproteins B100 and B48 were investigated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis to determine whether postprandially increased triglyceride is exogenous or endogenous. The level of apolipoproteins; A-I, B, C-II and E were increased in PHT rabbit after feeding. Apolipoproteins B100 and B48 were detected in the plasma fraction of d < 1.006 g/mL of the PHT rabbit. The postprandial increase in apolipoprotein B in the PHT rabbit reflects a numerical increase in lipoprotein particles in the blood; the increase in apolipoproteins C-II and E suggests some disturbance in lipoprotein catabolism. Apolipoprotein B48 was detected postprandially in PHT rabbits. These results suggest that delayed catabolism of exogenous lipids caused the retention of chylomicron remnants in the blood. Results also suggest that activities of the lipolytic enzyme lipoprotein lipase and hepatic triglyceride lipase were deficient and that the hepatic uptake of exogenous lipoproteins was delayed in the PHT rabbit. Especially, for examining remnant hyperlipoproteinaemia in humans, PHT rabbit is an excellent animal model for hypertriglyceridaemia research.