Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner
Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner
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DOI：
10.1016/j.febslet.2006.12.002
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发表时间：
2007-01-09
期刊：
影响因子：
3.5
通讯作者：
Ogawa, Yoshihiro
中科院分区：
文献类型：
--
作者：
Kamei, Yasutomi;Suganami, Takayoshi;Ogawa, Yoshihiro
父系表达1 (Peg1)/中胚层特异性转录本（Mest）是一种印迹基因，仅由父系等位基因转录而来。在一些人类癌症组织中，PEG1/MEST mRNA的选择性剪接变体使用不同的第一外显子的不同启动子，从父本和母本等位基因中表达。我们之前报道过Peg11 Mest在小鼠肥胖脂肪组织中的表达明显上调。此外，转基因Peg11Mest在脂肪组织中的过表达导致脂肪细胞体积增大。考虑到肥胖的潜在病理生理相关性，我们研究了肥胖脂肪组织中Peg11Mest表达增加的性质。在肥胖脂肪组织中，Peg11Mest的表达增加，而其他印迹基因的表达没有增加。肥胖脂肪组织中peg11est的转录率升高。我们发现小鼠Peg11Mest至少有四种同种异构体是通过使用可选的第一外显子产生的。我们还证明了在肥胖脂肪组织中大量表达的Peg11Mest保留了单等位基因表达。这是首次报道Peg11Mest在成人组织中的单等位基因诱导。(c) 2006年欧洲生化学会联合会。Elsevier B.V.版权所有。
Paternally expressed 1 (Peg1)/mesoderm specific transcript (Mest) is an imprinted gene, which is only transcribed from the paternal (father's) allele. In some human cancer tissues, an alternatively spliced variant of PEG1/MEST mRNA using a different promoter of a distinct first exon is expressed from both paternal and maternal alleles. We previously reported that Peg11 Mest expression was markedly up-regulated in obese adipose tissue in mice. Moreover, transgenic overexpression of Peg11Mest in the adipose tissue resulted in the enlargement of adipocytes in size. Given the potential pathophysiologic relevance in obesity, we examined the nature of increased expression of Peg11Mest in obese adipose tissue. In obese adipose tissue, expression of Peg11Mest was increased, but not that of other imprinted genes tested. The transcription rate of Peg11Mest was increased in obese adipose tissue. We found at least four isoforms of mouse Peg11Mest generated by use of the alternative first exons. We also demonstrated that the abundantly expressed Peg11Mest in obese adipose tissue retained monoallelic expression. This is the first report of monoallelic induction of Peg11Mest in adult tissues. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.