Quantitative Detection of Human Adenoviruses in Wastewater and Combined Sewer Overflows Influencing a Michigan River
Quantitative Detection of Human Adenoviruses in Wastewater and Combined Sewer Overflows Influencing a Michigan River
复制标题
DOI：
10.1128/aem.01316-09
复制
发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Rose, Joan B.
中科院分区：
文献类型：
--
作者：
Fong, Theng-Theng;Phanikumar, Mantha S.;Rose, Joan B.
肠道病毒是在受污染的地表沃茨中发现的重要病原体，以前曾在五大湖的沃茨中检测到过。对人类腺病毒进行监测是因为其在水生环境中的高流行率和持久性。在这项研究中，我们定量的腺病毒在废水，地表水，并结合下水道溢流（CSOs）的实时PCR。在2005年8月和2006年8月之间，在原污水，初级处理的污水，二级处理的污水和氯化污水在密歇根州的污水处理厂的腺病毒浓度进行了检查。CSO样品（n = 6）采集自大急流城（MI）的CSO保留池。在100%的废水和CSO排放样品中检测到腺病毒。污水和CSO中的平均腺病毒DNA浓度分别为1.15 × 10（6）病毒/升和5.35 × 10（5）病毒/升。腺病毒去除是
Enteric viruses are important pathogens found in contaminated surface waters and have previously been detected in waters of the Great Lakes. Human adenoviruses were monitored because of their high prevalence and persistence in aquatic environments. In this study, we quantified adenoviruses in wastewater, surface water, and combined sewer overflows (CSOs) by real-time PCR. Between August 2005 and August 2006, adenovirus concentrations in raw sewage, primary-treated effluent, secondary-treated effluent, and chlorinated effluent from a wastewater treatment plant in Michigan were examined. CSO samples (n = 6) were collected from a CSO retention basin in Grand Rapids, MI. Adenoviruses were detected in 100% of wastewater and CSO discharge samples. Average adenovirus DNA concentrations in sewage and CSOs were 1.15 x 10(6) viruses/liter and 5.35 x 10(5) viruses/liter, respectively. Adenovirus removal was